In this report, we describe the near-infrared fluorescent probes called quinaldine red (QR) is lit β-sheet structure of amyloid fibrils. Photochemical and biophysical properties of QR along with the other canonical probe in the presence of amyloid protein fibrils were investigated using fluorescence spectroscopy, confocal fluorescent microscopy and isothermal titration calorimetry.

Moreover, the binding sites and modes of interaction between QR and insulin fibrils were calculated based on molecular docking. Among these amyloid probe, QR show several advantages including a strong force supramolecular, near-infrared emission, high sensitivity and resistance to bleaching. A linear response of the fluorescence intensity of a sample QR nerve in the presence of sera visualized in the range of 1-30 pM, with a limit of detection (LOD) of 2.31 pM.

Recovery and relative standard deviation (RSD) of the proposed method for the determination of protein fibrils is 90.4% -99.2% and 3.05% -3.47%, respectively. Finally, QR can be fluorescently shine when meeting with aberrant protein aggregates of rat pathogens. We recommend the QR as a new and very good alternative to monitor the transition of the amyloid protein conformation.

A near-infrared fluorescent probe quinaldine red lights up the β-sheet structure of amyloid proteins in mouse brain.
A near-infrared fluorescent probe quinaldine red lights up the β-sheet structure of amyloid proteins in mouse brain.

Dark quantitative determination of pseudo Explains Low Chromophore Population Quantum Yield Red Fluorescent Protein.

The results of four representative fluorescence quantum red fluorescent protein mCherry, mKate2, mRuby2 and recently introduced mScarlet investigated. Durability excited state is measured as a function of the distance to the gold mirror to control the local density of optical states (LDOs). By analyzing the total emission rate as a function of LDOs, we obtain separately the emission level and the level of non-radiation of the sunny countries. Thus we obtain for the first time the results of quantum states of light protein uninterrupted dark, non-emitting state. Bright state quantum yields are much higher than previously reported results of quantum that the average for both bright and dark states.

We determine that mCherry, mKate2 and mRuby2 have a considerable fraction of darker chromophore up to 45%, which explains the low quantum results measured red emitting protein reported in the literature and the difficulty in developing a high quantum results of variants of the protein. To light recently developed mScarlet we find a small dark much smaller than 14%, accompanied by a very high quantum results from sunny countries 81%. The presence of largely dark chromophores have implications for a variety of fluorescent protein applications, ranging from quantitative fluorescence microscopy, FRET studies, to monitor protein expression levels.

We recommend that future optimization of the red fluorescent protein should pay more attention to minimize protein fractions dark. genetically encoded fluorescent protein (FP) are used in cell biology research to study the protein of interest or a signal process using a biosensor. To perform well in certain applications, it requires a lot of properties FP adjusted. It is for this reason that they need to be optimized by using mutagenesis. Process optimization through screening often based on the brightness of the colonies of bacteria, but some parameters ultimately determine the optimal performance of an FP.

Phycoerythrin (R-PE), affinity pure

GP-4077-30 1.0 mg
EUR 104.3

R Phycoerythrin antibody

70R-10657 1 mg
EUR 192
Description: Goat polyclonal R Phycoerythrin antibody

R Phycoerythrin antibody

70R-10691 1 mg
EUR 140
Description: Rabbit polyclonal R Phycoerythrin antibody

Phycoerythrin (R-PE) conjugated Streptadivin

AR-6635-01 1 ml
EUR 158.9

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-1 each
EUR 164.4

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-5 each
EUR 326.4

Phycoerythrin dye

85R-1018 1 mg
EUR 93
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies

Phycoerythrin (PE) Antibody Labeling Kit

20-abx098281
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Phycoerythrin antibody

10R-6521 50 ug
EUR 165
Description: Mouse monoclonal Phycoerythrin antibody

Phycoerythrin B Antibody

GWB-305CC1 1 mg Ask for price

Phycoerythrin B Antibody

GWB-B71E19 1 ml Ask for price

Phycoerythrin antibody (biotin)

61R-1583 100 ug
EUR 258
Description: Mouse monoclonal Phycoerythrin antibody (biotin)

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Phycoerythrin (PE)

TA150020 100 µl Ask for price

Mouse monoclonal anti-His Antibody, Clone OTI2B5, Phycoerythrin (PE)

TA150130 100 µl Ask for price

Phycoerythrin (PE) conjugated anti-SAM monoclonal antibody Clone 84-3

MAF00202-50 50µl
EUR 690
Description: Phycoerythrin (PE) conjugated anti-SAM monoclonal antibody Clone 84-3

Anti-BIOTIN (Goat) Phycoerythrin Conjugated Antibody

GWB-EC5452 1 mL Ask for price

OORA00190-1ML - STREPTAVIDIN PHYCOERYTHRIN Conjugated

OORA00190-1ML 1mL
EUR 379

Mouse Monoclonal Anti-Human IgG1 (Fc-region)-PE (phycoerythrin) conjugate

10121-PE 0.5 ml
EUR 489.6

Mouse Monoclonal Anti-Human IgG2 (Fc-region)-PE (phycoerythrin) conjugate

10122-PE 0.5 ml
EUR 489.6

Mouse Monoclonal Anti-Human IgG4 (Fc-region)-PE (phycoerythrin) conjugate

10124-PE 0.5 ml
EUR 489.6

Right Reference Standard™ Phycoerythrin High (100 tests)

BLI515-5 5ml
EUR 444

Mouse Monoclonal Anti-Human IgG3 (Hinge-region)-PE (phycoerythrin) conjugate

10123-PE 0.5 ml
EUR 489.6

Goat Anti-Rat IgG (H+L)-Phycoerythrin (PE) Conjugate (adsorbed with other species)

50332 0.5 ml
EUR 300

Goat Anti-Human IgG (H+L)-Phycoerythrin (PE) Conjugate (adsorbed with other species)

10332 0.5 ml
EUR 300

Phycoerythrin Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SP005 250 µg
EUR 255

Phycoerythrin Conjugated Affinity Purified anti-Human IgG [H&L] [Goat]

SP002 250 µg
EUR 255

Phycoerythrin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]

SP003 250 µg
EUR 255

Phycoerythrin Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]

SP001 250 µg
EUR 255

Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SP004 250 µg
EUR 255

Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins)

GWB-878BA0 1 mL Ask for price

OARA04577-v1 - F(ab')2 Anti-RAT IgG F(ab')2 (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hs & Hu Serum Proteins (1ml)

OARA04577-v1 1ml
EUR 480

Phycoerythrin Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT) (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins)

GWB-B20D13 1 mL Ask for price

OARA04567-v1 - F(ab')2 Anti-HUMAN IgM Fc5u (DONKEY) Antibody Phycoerythrin conjugated Min X Bv & Hs Serum Proteins (1ml)

OARA04567-v1 1ml
EUR 480

OARA04577-1ML - F(ab')2 Anti-RAT IgG F(ab')2 (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hs & Hu Serum Proteins (1ml)

OARA04577-1ML 1mL
EUR 439

OARA04567-1ML - F(ab')2 Anti-HUMAN IgM Fc5u (DONKEY) Antibody Phycoerythrin conjugated Min X Bv & Hs Serum Proteins (1ml)

OARA04567-1ML 1mL
EUR 439

Phycoerythrin Conjugated Affinity Purified anti-Mouse IgG [H&L] (Goat) Min X Bovine Chicken Goat Guinea Pig Horse Hamster Human

GWB-80A0F4 1 mL Ask for price

Red Fluorescent Protein (RFP) Antibody

abx412396-50ug 50 ug
EUR 678

Red Fluorescent Protein (RFP) Antibody

abx240273-100l 100 µl Ask for price

Red Fluorescent Protein (RFP) Antibody

abx240273-50l 50 µl
EUR 350

Red Fluorescent Protein (RFP) Antibody

abx412396-100g 100 µg
EUR 450

Red Fluorescent Protein (dsRed) Antibody

3994-100 each
EUR 451.2

Anti-RFP (Red Fluorescent Protein) Antibody

GWB-3BF397 100 ug Ask for price

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody

ABP-MAB-RT008 100 ug Ask for price

Anti-GFP (Green Fluorescent Protein) (Goat) Texas Red Conjugated

GWB-2921F1 1 mg Ask for price

Fluorescent Protein Set

K816-6-100 each
EUR 1488

Anti-GFP (Green Fluorescent Protein) (Mouse) Texas Red Conjugated Antibody

GWB-D2D74D 1 mg Ask for price

Cyan Fluorescent Protein

30R-2808 100 ug
EUR 462
Description: Purified recombinant Human Cyan Fluorescent Protein

Cyan Fluorescent Protein

4996-100 each
EUR 451.2

Cyan Fluorescent Protein

4996-1000 each
EUR 2865.6

Cyan Fluorescent Protein

4996-5000 each
EUR 7824

7-AAD red fluorescent vital stain

6163 125 tests
EUR 172

dsRed Fluorescent protein

30R-2809 100 ug
EUR 462
Description: Purified recombinant Human dsRed Fluorescent protein

dsRed Fluorescent Protein

4997-100 each
EUR 489.6

dsRed Fluorescent Protein

4997-1000 each
EUR 3008.4

dsRed Fluorescent Protein

4997-5000 each
EUR 8626.8

Green Fluorescent Protein

CH22124 100 ul of 1 mg/ml
EUR 522

Green Fluorescent Protein

MO22184 100 ul
EUR 490.8

Protein G Fluorescent Particles, Nile Red, 0.1%w/v, 0.4-0.6µm, 5mL

PGFP-0556-5 5 mL
EUR 133
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Yellow Fluorescent Protein

4998-100 each
EUR 470.4

Yellow Fluorescent Protein

4998-1000 each
EUR 3015.6

Yellow Fluorescent Protein

4998-5000 each
EUR 8215.2

YFP-P2A-LOCa3 (coexpressing LOCa3, mKate2 a red fluorescent protein as marker)

AX0010033 1.0 µg DNA
EUR 245

mCherry Fluorescent Protein

4993-100 each
EUR 489.6

mCherry Fluorescent Protein

4993-1000 each
EUR 3145.2

mCherry Fluorescent Protein

4993-5000 each
EUR 9175.2

Recombinant purified Red Fluorescent Proteins (RFP/dsRed) protein control for Western blot

RFP12-C 100 ul
EUR 343.2

mKate2-P2A-LOCa3 (coexpressing LOCa3, mKate2 a red fluorescent protein as marker)

AX0010032 1.0 µg DNA
EUR 245

Blue Fluorescent Protein (BFP)

4994-100 each
EUR 451.2

Blue Fluorescent Protein (BFP)

4994-1000 each
EUR 2871.6

Blue Fluorescent Protein (BFP)

4994-5000 each
EUR 8353.2

Recombinant (E. coli) Red Fluorescent Proteins (RFP/dsRed) protein for ELISA or Standards (>98%)

RFP15-R 25 ug
EUR 300

Green Fluorescent Protein (GFP)

CH23105 400 ul
EUR 913.2

Green Fluorescent Protein (GFP)

MO22190 100 ul
EUR 490.8

Green Fluorescent Protein (GFP)

RA22134 100 ul of 1 mg/ml
EUR 490.8

Green Fluorescent Protein (GFP) Protein

20-abx651492
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Green Fluorescent Protein (GFP) Protein

abx260632-10g 10 µg
EUR 325

Green Fluorescent Protein (GFP) Protein

abx260632-2g 2 µg
EUR 225

Green Fluorescent Protein (GFP) Protein

abx651492-5mg 5 mg
EUR 475

Protein A Coated Fluorescent Particles, Nile Red, 0.1%w/v, 0.4-0.6µm, 5mL

PAFP-0556-5 5 mL
EUR 115.5
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Protein Antibody Set

K817-6-100 each
EUR 1488

Fluorescent Particle Slide, Nile Red, 10.0-14.0µm

FPS-10056 mL
EUR 98
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Green Fluorescent Protein Antibody

GWB-21E341 1 ml Ask for price

Green Fluorescent Protein Antibody

GWB-2DAA70 0.1 ml Ask for price

Green Fluorescent Protein Antibody

GWB-A6ABAF 0.25 mg Ask for price

Green Fluorescent protein Antibody

GWB-6A6C1C 0.1 mg Ask for price

Green Fluorescent Protein Antibody

GWB-FF1300 0.1 mg Ask for price

Green Fluorescent Protein Antibody

GWB-B77B66 0.05 mg Ask for price

Green Fluorescent Protein Antibody

GWB-B8A68D 0.1 mg Ask for price

Blue Fluorescent protein (His tag)

80R-2547 100 ug
EUR 447
Description: Purified recombinant Human Blue Fluorescent protein

TruStrip WBV Red Fluorescent protein (RFP) quantitation & Western Blot Validation Strips (10/pk)

WBRFP10-10 1 Pk
EUR 270

Cherry Fluorescent Protein (His tag)

80R-2546 100 ug
EUR 475
Description: Purified recombinant Human Cherry Fluorescent Protein

Green Fluorescent Protein (GFP), 25UG

L003-25UG 25UG
EUR 424.8

Absolute Mag™ Protein G Magnetic Polystyrene Particles, Red Fluorescent, 2 µm

WHM-G272 2 ml
EUR 1122

Recombinant Green Fluorescent Protein

7-06112 10µg Ask for price

Recombinant Green Fluorescent Protein

7-06113 50µg Ask for price

Recombinant Green Fluorescent Protein

7-06114 1mg Ask for price

Green Fluorescent Protein Recombinant

rAP-4425 Inquiry Ask for price

Green Fluorescent Protein ELISA Kit

ECP7863 96 Tests
EUR 646

Fluorescent Protein Marker (14-200kDa)

MKP004-250mL 250μL
EUR 42

Flow Check™ Ruby Red Fluorescent Microspheres

24288-5 5ml
EUR 338

DiagNano Red Fluorescent Silica Particles, 300 nm

DNG-L020 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 500 nm

DNG-L025 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 1 μm

DNG-L026 10 mL
EUR 758.4

DiagNano Red Fluorescent Silica Particles, 1.5 μm

DNG-L029 10 mL
EUR 758.4

DiagNano Red Fluorescent Silica Particles, 3 μm

DNG-L032 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 5 μm

DNG-L033 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 10 μm

DNG-L036 10 mL
EUR 764.4

DiagNano Red Fluorescent Silica Particles, 15 μm

DNG-L038 10 mL
EUR 764.4

DiagNano Red Fluorescent Silica Particles, 20 μm

DNG-L039 10 mL
EUR 764.4

Fluorescent Particle Slide, Nile Red, 5.0-5.9µm

FPS-5056 mL
EUR 98
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Absolute Mag Protein G Magnetic Polystyrene particles, Fluorescent Red, 2 µm

WHM-M079 1 mL
EUR 945.6

Blue Fluorescent Protein (BFP) Antibody

abx412394-50ug 50 ug
EUR 594

Cyan Fluorescent Protein (CFP) Antibody

abx412395-50ug 50 ug
EUR 594

Blue Fluorescent Protein (BFP) Antibody

abx412394-100tests 100 tests
EUR 375

Green Fluorescent Protein (GFP) Polyclonal Antibody (Pan-species), PE

4-PAD025Ge07-PE
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Description: A Rabbit polyclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with PE.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), PE

4-MAD025Ge21-PE
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with PE.

Green Fluorescent Protein (GFP) Antibody

20-abx118007
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Green Fluorescent Protein (GFP) Antibody

abx140045-01mg 0.1 mg
EUR 460.8

Green Fluorescent Protein (GFP) Antibody

20-abx132159
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Green Fluorescent Protein (GFP) Antibody

20-abx134154
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Green Fluorescent Protein (GFP) Antibody

20-abx134155
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Green Fluorescent Protein (GFP) Antibody

20-abx134156
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Green Fluorescent Protein (GFP) Antibody

20-abx134157
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Green Fluorescent Protein (GFP) Antibody

20-abx137422
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Green Fluorescent Protein (GFP) Antibody

20-abx005584
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Green Fluorescent Protein (GFP) Antibody

20-abx005585
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Green Fluorescent Protein (GFP) Antibody

abx034623-400ul 400 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody

abx034623-80l 80 µl
EUR 343.2

Green Fluorescent Protein (GFP) Antibody

abx034624-100ul 100 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody

abx037713-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody

abx037714-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody

abx015749-100ul 100 ul
EUR 493.2

Green Fluorescent Protein (GFP) Antibody

abx019093-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody

abx019094-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody

abx019099-100ug 100 ug
EUR 410.4

Green Fluorescent Protein (GFP) Antibody

20-abx020016
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Green Fluorescent Protein (GFP) Antibody

20-abx210090
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Green Fluorescent Protein (GFP) Antibody

20-abx210351
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Green Fluorescent Protein (GFP) Antibody

abx233433-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

abx233434-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

abx233435-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

20-abx159519
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Green Fluorescent Protein (GFP), Antibody

GWB-35A90C 0.1 mg Ask for price

Green Fluorescent Protein (GFP), Antibody

GWB-6E6630 1 mg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx011187-100g 100 µg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx011187-10g 10 µg
EUR 350

Green Fluorescent Protein (GFP) Antibody

abx011187-200g 200 µg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx005584-100g 100 µg
EUR 200

Green Fluorescent Protein (GFP) Antibody

abx005585-100l 100 µl
EUR 200

Green Fluorescent Protein (GFP) Antibody

abx159519-100g 100 µg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx159519-10g 10 µg
EUR 187.5

Green Fluorescent Protein (GFP) Antibody

abx159519-50g 50 µg
EUR 250

Green Fluorescent Protein (GFP) Antibody

abx118007-100l 100 µl Ask for price

Green Fluorescent Protein (GFP) Antibody

abx118007-20l 20 µl
EUR 287.5

Green Fluorescent Protein (GFP) Antibody

abx118007-50l 50 µl
EUR 362.5

Green Fluorescent Protein (GFP) Antibody

abx210090-100l 100 µl
EUR 275

Green Fluorescent Protein (GFP) Antibody

abx210090-50l 50 µl
EUR 212.5

Instead of the selection of the characteristics of other properties, we develop a multiparameter method of screening based on four critical parametersscreened simultaneously: fluorescence lifetime, brightness cellular maturation efficiency and photostability.