In this report, we describe the near-infrared fluorescent probes called quinaldine red (QR) is lit β-sheet structure of amyloid fibrils. Photochemical and biophysical properties of QR along with the other canonical probe in the presence of amyloid protein fibrils were investigated using fluorescence spectroscopy, confocal fluorescent microscopy and isothermal titration calorimetry.
Moreover, the binding sites and modes of interaction between QR and insulin fibrils were calculated based on molecular docking. Among these amyloid probe, QR show several advantages including a strong force supramolecular, near-infrared emission, high sensitivity and resistance to bleaching. A linear response of the fluorescence intensity of a sample QR nerve in the presence of sera visualized in the range of 1-30 pM, with a limit of detection (LOD) of 2.31 pM.
Recovery and relative standard deviation (RSD) of the proposed method for the determination of protein fibrils is 90.4% -99.2% and 3.05% -3.47%, respectively. Finally, QR can be fluorescently shine when meeting with aberrant protein aggregates of rat pathogens . We recommend the QR as a new and very good alternative to monitor the transition of the amyloid protein conformation.
A near-infrared fluorescent probe quinaldine red lights up the β-sheet structure of amyloid proteins in mouse brain.
Dark quantitative determination of pseudo Explains Low Chromophore Population Quantum Yield Red Fluorescent Protein.
The results of four representative fluorescence quantum red fluorescent protein mCherry, mKate2, mRuby2 and recently introduced mScarlet investigated. Durability excited state is measured as a function of the distance to the gold mirror to control the local density of optical states (LDOs). By analyzing the total emission rate as a function of LDOs, we obtain separately the emission level and the level of non-radiation of the sunny countries. Thus we obtain for the first time the results of quantum states of light protein uninterrupted dark, non-emitting state. Bright state quantum yields are much higher than previously reported results of quantum that the average for both bright and dark states.
We determine that mCherry, mKate2 and mRuby2 have a considerable fraction of darker chromophore up to 45%, which explains the low quantum results measured red emitting protein reported in the literature and the difficulty in developing a high quantum results of variants of the protein. To light recently developed mScarlet we find a small dark much smaller than 14%, accompanied by a very high quantum results from sunny countries 81%. The presence of largely dark chromophores have implications for a variety of fluorescent protein applications, ranging from quantitative fluorescence microscopy, FRET studies, to monitor protein expression levels.
We recommend that future optimization of the red fluorescent protein should pay more attention to minimize protein fractions dark. genetically encoded fluorescent protein (FP) are used in cell biology research to study the protein of interest or a signal process using a biosensor. To perform well in certain applications, it requires a lot of properties FP adjusted. It is for this reason that they need to be optimized by using mutagenesis. Process optimization through screening often based on the brightness of the colonies of bacteria, but some parameters ultimately determine the optimal performance of an FP.
Phycoerythrin (R-PE), affinity pure
GP-4077-30
ImmunoBioscience
1.0 mg
EUR 104.3
R Phycoerythrin antibody
70R-10657
Fitzgerald
1 mg
EUR 192
Description: Goat polyclonal R Phycoerythrin antibody
R Phycoerythrin antibody
70R-10691
Fitzgerald
1 mg
EUR 140
Description: Rabbit polyclonal R Phycoerythrin antibody
Phycoerythrin (R-PE) conjugated Streptadivin
AR-6635-01
ImmunoBioscience
1 ml
EUR 158.9
Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-1
Biovision
each
EUR 164.4
Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-5
Biovision
each
EUR 326.4
Phycoerythrin dye
85R-1018
Fitzgerald
1 mg
EUR 93
Description: Phycocyanin dye for use in conjugation to biomolecules and antibodies
Phycoerythrin (PE) Antibody Labeling Kit
20-abx098281
Abbexa
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Phycoerythrin antibody
10R-6521
Fitzgerald
50 ug
EUR 165
Description: Mouse monoclonal Phycoerythrin antibody
Phycoerythrin antibody (biotin)
61R-1583
Fitzgerald
100 ug
EUR 258
Description: Mouse monoclonal Phycoerythrin antibody (biotin)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Phycoerythrin (PE)
TA150020
Origene Technologies GmbH
100 µl
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Phycoerythrin (PE) conjugated anti-SAM monoclonal antibody Clone 84-3
MAF00202-50
Arthus
50µl
EUR 690
Description: Phycoerythrin (PE) conjugated anti-SAM monoclonal antibody Clone 84-3
Anti-BIOTIN (Goat) Phycoerythrin Conjugated Antibody
GWB-EC5452
GenWay Biotech
1 mL
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Mouse Monoclonal Anti-Human IgG1 (Fc-region)-PE (phycoerythrin) conjugate
10121-PE
Alpha Diagnostics
0.5 ml
EUR 489.6
Mouse Monoclonal Anti-Human IgG2 (Fc-region)-PE (phycoerythrin) conjugate
10122-PE
Alpha Diagnostics
0.5 ml
EUR 489.6
Mouse Monoclonal Anti-Human IgG4 (Fc-region)-PE (phycoerythrin) conjugate
10124-PE
Alpha Diagnostics
0.5 ml
EUR 489.6
Mouse Monoclonal Anti-Human IgG3 (Hinge-region)-PE (phycoerythrin) conjugate
10123-PE
Alpha Diagnostics
0.5 ml
EUR 489.6
Goat Anti-Rat IgG (H+L)-Phycoerythrin (PE) Conjugate (adsorbed with other species)
50332
Alpha Diagnostics
0.5 ml
EUR 300
Goat Anti-Human IgG (H+L)-Phycoerythrin (PE) Conjugate (adsorbed with other species)
10332
Alpha Diagnostics
0.5 ml
EUR 300
Phycoerythrin Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]
SP005
ABM
250 µg
EUR 255
Phycoerythrin Conjugated Affinity Purified anti-Human IgG [H&L] [Goat]
SP002
ABM
250 µg
EUR 255
Phycoerythrin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]
SP003
ABM
250 µg
EUR 255
Phycoerythrin Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]
SP001
ABM
250 µg
EUR 255
Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]
SP004
ABM
250 µg
EUR 255
Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins)
GWB-878BA0
GenWay Biotech
1 mL
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OARA04577-v1 - F(ab')2 Anti-RAT IgG F(ab')2 (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hs & Hu Serum Proteins (1ml)
OARA04577-v1
Aviva Systems Biology
1ml
EUR 480
Phycoerythrin Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT) (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins)
GWB-B20D13
GenWay Biotech
1 mL
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OARA04567-v1 - F(ab')2 Anti-HUMAN IgM Fc5u (DONKEY) Antibody Phycoerythrin conjugated Min X Bv & Hs Serum Proteins (1ml)
OARA04567-v1
Aviva Systems Biology
1ml
EUR 480
OARA04577-1ML - F(ab')2 Anti-RAT IgG F(ab')2 (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hs & Hu Serum Proteins (1ml)
OARA04577-1ML
Aviva Systems Biology
1mL
EUR 439
OARA04567-1ML - F(ab')2 Anti-HUMAN IgM Fc5u (DONKEY) Antibody Phycoerythrin conjugated Min X Bv & Hs Serum Proteins (1ml)
OARA04567-1ML
Aviva Systems Biology
1mL
EUR 439
Phycoerythrin Conjugated Affinity Purified anti-Mouse IgG [H&L] (Goat) Min X Bovine Chicken Goat Guinea Pig Horse Hamster Human
GWB-80A0F4
GenWay Biotech
1 mL
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Red Fluorescent Protein (RFP) Antibody
abx412396-50ug
Abbexa
50 ug
EUR 678
Red Fluorescent Protein (RFP) Antibody
abx240273-100l
Abbexa
100 µl
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Red Fluorescent Protein (RFP) Antibody
abx240273-50l
Abbexa
50 µl
EUR 350
Red Fluorescent Protein (RFP) Antibody
abx412396-100g
Abbexa
100 µg
EUR 450
Red Fluorescent Protein (dsRed) Antibody
3994-100
Biovision
each
EUR 451.2
Anti-RFP (Red Fluorescent Protein) Antibody
GWB-3BF397
GenWay Biotech
100 ug
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Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
ABP-MAB-RT008
Allele Biotech
100 ug
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Anti-GFP (Green Fluorescent Protein) (Goat) Texas Red Conjugated
GWB-2921F1
GenWay Biotech
1 mg
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Fluorescent Protein Set
K816-6-100
Biovision
each
EUR 1488
Anti-GFP (Green Fluorescent Protein) (Mouse) Texas Red Conjugated Antibody
GWB-D2D74D
GenWay Biotech
1 mg
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Cyan Fluorescent Protein
30R-2808
Fitzgerald
100 ug
EUR 462
Description: Purified recombinant Human Cyan Fluorescent Protein
Cyan Fluorescent Protein
4996-100
Biovision
each
EUR 451.2
Cyan Fluorescent Protein
4996-1000
Biovision
each
EUR 2865.6
Cyan Fluorescent Protein
4996-5000
Biovision
each
EUR 7824
dsRed Fluorescent protein
30R-2809
Fitzgerald
100 ug
EUR 462
Description: Purified recombinant Human dsRed Fluorescent protein
dsRed Fluorescent Protein
4997-100
Biovision
each
EUR 489.6
dsRed Fluorescent Protein
4997-1000
Biovision
each
EUR 3008.4
dsRed Fluorescent Protein
4997-5000
Biovision
each
EUR 8626.8
Green Fluorescent Protein
CH22124
Neuromics
100 ul of 1 mg/ml
EUR 522
Green Fluorescent Protein
MO22184
Neuromics
100 ul
EUR 490.8
Protein G Fluorescent Particles, Nile Red, 0.1%w/v, 0.4-0.6µm, 5mL
PGFP-0556-5
Spherotech
5 mL
EUR 133
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Yellow Fluorescent Protein
4998-100
Biovision
each
EUR 470.4
Yellow Fluorescent Protein
4998-1000
Biovision
each
EUR 3015.6
Yellow Fluorescent Protein
4998-5000
Biovision
each
EUR 8215.2
YFP-P2A-LOCa3 (coexpressing LOCa3, mKate2 a red fluorescent protein as marker)
AX0010033
ABM
1.0 µg DNA
EUR 245
mCherry Fluorescent Protein
4993-100
Biovision
each
EUR 489.6
mCherry Fluorescent Protein
4993-1000
Biovision
each
EUR 3145.2
mCherry Fluorescent Protein
4993-5000
Biovision
each
EUR 9175.2
Recombinant purified Red Fluorescent Proteins (RFP/dsRed) protein control for Western blot
RFP12-C
Alpha Diagnostics
100 ul
EUR 343.2
mKate2-P2A-LOCa3 (coexpressing LOCa3, mKate2 a red fluorescent protein as marker)
AX0010032
ABM
1.0 µg DNA
EUR 245
Blue Fluorescent Protein (BFP)
4994-100
Biovision
each
EUR 451.2
Blue Fluorescent Protein (BFP)
4994-1000
Biovision
each
EUR 2871.6
Blue Fluorescent Protein (BFP)
4994-5000
Biovision
each
EUR 8353.2
Recombinant (E. coli) Red Fluorescent Proteins (RFP/dsRed) protein for ELISA or Standards (>98%)
RFP15-R
Alpha Diagnostics
25 ug
EUR 300
Green Fluorescent Protein (GFP)
CH23105
Neuromics
400 ul
EUR 913.2
Green Fluorescent Protein (GFP)
MO22190
Neuromics
100 ul
EUR 490.8
Green Fluorescent Protein (GFP)
RA22134
Neuromics
100 ul of 1 mg/ml
EUR 490.8
Green Fluorescent Protein (GFP) Protein
20-abx651492
Abbexa
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100 ug
10 ug
1 mg
200 ug
50 ug
Green Fluorescent Protein (GFP) Protein
abx260632-10g
Abbexa
10 µg
EUR 325
Green Fluorescent Protein (GFP) Protein
abx260632-2g
Abbexa
2 µg
EUR 225
Green Fluorescent Protein (GFP) Protein
abx651492-5mg
Abbexa
5 mg
EUR 475
Protein A Coated Fluorescent Particles, Nile Red, 0.1%w/v, 0.4-0.6µm, 5mL
PAFP-0556-5
Spherotech
5 mL
EUR 115.5
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Fluorescent Protein Antibody Set
K817-6-100
Biovision
each
EUR 1488
Fluorescent Particle Slide, Nile Red, 10.0-14.0µm
FPS-10056
Spherotech
mL
EUR 98
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Green Fluorescent Protein Antibody
GWB-21E341
GenWay Biotech
1 ml
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Green Fluorescent Protein Antibody
GWB-2DAA70
GenWay Biotech
0.1 ml
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Green Fluorescent Protein Antibody
GWB-A6ABAF
GenWay Biotech
0.25 mg
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Green Fluorescent protein Antibody
GWB-6A6C1C
GenWay Biotech
0.1 mg
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Green Fluorescent Protein Antibody
GWB-FF1300
GenWay Biotech
0.1 mg
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Green Fluorescent Protein Antibody
GWB-B77B66
GenWay Biotech
0.05 mg
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Green Fluorescent Protein Antibody
GWB-B8A68D
GenWay Biotech
0.1 mg
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Blue Fluorescent protein (His tag)
80R-2547
Fitzgerald
100 ug
EUR 447
Description: Purified recombinant Human Blue Fluorescent protein
TruStrip WBV Red Fluorescent protein (RFP) quantitation & Western Blot Validation Strips (10/pk)
WBRFP10-10
Alpha Diagnostics
1 Pk
EUR 270
Cherry Fluorescent Protein (His tag)
80R-2546
Fitzgerald
100 ug
EUR 475
Description: Purified recombinant Human Cherry Fluorescent Protein
Green Fluorescent Protein (GFP), 25UG
L003-25UG
Arbor Assays
25UG
EUR 424.8
Absolute Mag™ Protein G Magnetic Polystyrene Particles, Red Fluorescent, 2 µm
WHM-G272
Creative Diagnostics
2 ml
EUR 1122
Recombinant Green Fluorescent Protein
7-06112
CHI Scientific
10µg
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Recombinant Green Fluorescent Protein
7-06113
CHI Scientific
50µg
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Recombinant Green Fluorescent Protein
7-06114
CHI Scientific
1mg
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Green Fluorescent Protein Recombinant
rAP-4425
Angio Proteomie
Inquiry
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Green Fluorescent Protein ELISA Kit
ECP7863
Genovis AB
96 Tests
EUR 646
Fluorescent Particle Slide, Nile Red, 5.0-5.9µm
FPS-5056
Spherotech
mL
EUR 98
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Absolute Mag Protein G Magnetic Polystyrene particles, Fluorescent Red, 2 µm
WHM-M079
Creative Diagnostics
1 mL
EUR 945.6
Blue Fluorescent Protein (BFP) Antibody
abx412394-50ug
Abbexa
50 ug
EUR 594
Cyan Fluorescent Protein (CFP) Antibody
abx412395-50ug
Abbexa
50 ug
EUR 594
Blue Fluorescent Protein (BFP) Antibody
abx412394-100tests
Abbexa
100 tests
EUR 375
Green Fluorescent Protein (GFP) Polyclonal Antibody (Pan-species), PE
4-PAD025Ge07-PE
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Rabbit polyclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with PE.
Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), PE
4-MAD025Ge21-PE
Cloud-Clone
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100ul
10ml
1ml
200ul
20ul
Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with PE.
Green Fluorescent Protein (GFP) Antibody
20-abx118007
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx140045-01mg
Abbexa
0.1 mg
EUR 460.8
Green Fluorescent Protein (GFP) Antibody
20-abx132159
Abbexa
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100 ug
10 ug
1 mg
200 ug
50 ug
Green Fluorescent Protein (GFP) Antibody
20-abx134154
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx134155
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx134156
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx134157
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx137422
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx005584
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx005585
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx034623-400ul
Abbexa
400 ul
EUR 627.6
Green Fluorescent Protein (GFP) Antibody
abx034623-80l
Abbexa
80 µl
EUR 343.2
Green Fluorescent Protein (GFP) Antibody
abx034624-100ul
Abbexa
100 ul
EUR 627.6
Green Fluorescent Protein (GFP) Antibody
abx037713-100ug
Abbexa
100 ug
EUR 469.2
Green Fluorescent Protein (GFP) Antibody
abx037714-100ug
Abbexa
100 ug
EUR 469.2
Green Fluorescent Protein (GFP) Antibody
abx015749-100ul
Abbexa
100 ul
EUR 493.2
Green Fluorescent Protein (GFP) Antibody
abx019093-100ug
Abbexa
100 ug
EUR 385.2
Green Fluorescent Protein (GFP) Antibody
abx019094-100ug
Abbexa
100 ug
EUR 385.2
Green Fluorescent Protein (GFP) Antibody
abx019099-100ug
Abbexa
100 ug
EUR 410.4
Green Fluorescent Protein (GFP) Antibody
20-abx020016
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx210090
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx210351
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx233433-100ug
Abbexa
100 ug
EUR 577.2
Green Fluorescent Protein (GFP) Antibody
abx233434-100ug
Abbexa
100 ug
EUR 577.2
Green Fluorescent Protein (GFP) Antibody
abx233435-100ug
Abbexa
100 ug
EUR 577.2
Green Fluorescent Protein (GFP) Antibody
20-abx159519
Abbexa
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Green Fluorescent Protein (GFP), Antibody
GWB-35A90C
GenWay Biotech
0.1 mg
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Green Fluorescent Protein (GFP), Antibody
GWB-6E6630
GenWay Biotech
1 mg
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Green Fluorescent Protein (GFP) Antibody
abx011187-100g
Abbexa
100 µg
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Green Fluorescent Protein (GFP) Antibody
abx011187-10g
Abbexa
10 µg
EUR 350
Green Fluorescent Protein (GFP) Antibody
abx011187-200g
Abbexa
200 µg
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Green Fluorescent Protein (GFP) Antibody
abx005584-100g
Abbexa
100 µg
EUR 200
Green Fluorescent Protein (GFP) Antibody
abx005585-100l
Abbexa
100 µl
EUR 200
Green Fluorescent Protein (GFP) Antibody
abx159519-100g
Abbexa
100 µg
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Green Fluorescent Protein (GFP) Antibody
abx159519-10g
Abbexa
10 µg
EUR 187.5
Green Fluorescent Protein (GFP) Antibody
abx159519-50g
Abbexa
50 µg
EUR 250
Green Fluorescent Protein (GFP) Antibody
abx118007-100l
Abbexa
100 µl
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Green Fluorescent Protein (GFP) Antibody
abx118007-20l
Abbexa
20 µl
EUR 287.5
Green Fluorescent Protein (GFP) Antibody
abx118007-50l
Abbexa
50 µl
EUR 362.5
Green Fluorescent Protein (GFP) Antibody
abx210090-100l
Abbexa
100 µl
EUR 275
Green Fluorescent Protein (GFP) Antibody
abx210090-50l
Abbexa
50 µl
EUR 212.5
Instead of the selection of the characteristics of other properties, we develop a multiparameter method of screening based on four critical parametersscreened simultaneously: fluorescence lifetime, brightness cellular maturation efficiency and photostability.