The endoplasmic reticulum (ER) is a multifunctional organelles that perform multiple cellular activities in eukaryotes. ER visualize using fluorescent protein is a powerful method to analyze the dynamics and understanding its function. However, the second red fluorescent protein with N-terminal signal peptide (SP) and ER retention of the C-terminal tetrapeptide (HDEL) often cause mislocalization into the vacuole or the extracellular space when they are constitutively expressed in Arabidopsis.

To obtain red fluorescent markers ER, we chose Arabidopsis cytochrome b5 -B (CB5-B), a (TA) proteins tails anchored in the ER membrane. localization is determined by the transmembrane domain (TMD) and the tail domain at the C-terminus. We fused tail domain TMD and CB5-B to the C-terminus of the red fluorescent protein, tdTomato (tdTomato-CTT).

When tdTomato-CTT was under the constitutive promoter ubiquitin10 in Arabidopsis, a fluorescent signal is detected exclusively in the ER by means of ER marker SP-GFP-HDEL reliable. Therefore, tdTomato-CTT can accurately visualize ER in Arabidopsis line stable. In addition, while tests show that tdTomato-CTT also be used as an ER marker in onions, rice, and Nicotiana benthamiana. We believe that the TA protein could be used to produce a variety of membrane markers organellar in plants.

 Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b 5 -B
Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b 5 -B

Supramolecular Small Encapsulation-Ultra Red Fluorescent Protein in Virus-Like Nanoparticles for Non-Invasive In Vivo Imaging Agents.

icosahedral virus-like particle (VLP) derived from bacteriophage Qβ and PP7 encapsulating ultra small red fluorescent protein (smURFP) is manufactured using a versatile supramolecualr capsid-dissassemble approach regroup. VLP produced neon displays structural properties identical to the non-fluorescent analog them.

Encapsulated smURFP show photochemical properties distinguished by its unencapsulated colleagues, showed remarkable stability against pH, and produce bright images following in vitro phagocytosis by macrophages. In vivo imaging allows biodistribution to be imaged at the points at different times. Ex vivo imaging of intravenous packed smURFP reveleas localization in the liver and kidneys after 2 hours of blood circulation and the elimination of substantial construction as a non-invasive in vivo imaging agents.


Genetically encoded Forster Resonance Energy Transfer (FRET) based biosensor is a powerful tool to illuminate the spatiotemporal regulation of cell signaling in living cells, but the utility of the red spectrum for biosensing limited due to the lack of light and stable red fluorescent protein. Here, we are rational increase photophysical characteristics of coral-derived fluorescent protein TagRFP-T. We showed that the mutant residue new, super-TagRFP (stagRFP) has almost twice the brightness of molecules TagRFP-T and photoactivation ignored. stagRFP facilitate significant improvements on a few green-red biosensor as FRET acceptor and a donor efficient fret that supports the red / far-red fret biosensing.

Rainbow Fluorescent Particles

RFP-20-5 5 mL
EUR 294
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles

RFP-30-5 5 mL
EUR 304
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles

RFP-30-5A 5X1 mL
EUR 304
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles

RFP-35-5 5 mL
EUR 304
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles

RFP-38-5 5 mL
EUR 157
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles

RFP-50-5 5 mL
EUR 314
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles

RFP-60-5 5 mL
EUR 314
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Rainbow Fluorescent Particles

RFP-70-5 5 mL
EUR 314
Description: Rainbow Fluorescent Particlesare calibration particles that help you to determine if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO? Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-1
EUR 137

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-5
EUR 272

Recombinant purified Red Fluorescent Proteins (RFP/dsRed) protein control for Western blot

RFP12-C 100 ul
EUR 286

EZ-Red? E. coli Fluorescent Particles

M1202-500
EUR 343

EZ-Red? Zymosan A Fluorescent Particles

M1204-500
EUR 343

RFP Expressing Human Glioblastoma Cells

TR01-RFP 500,000 Cells
EUR 1354

TruStrip WBV Red Fluorescent protein (RFP) quantitation & Western Blot Validation Strips (10/pk)

WBRFP10-10 1 Pk
EUR 225

DiagNano Red Fluorescent Silica Nanoparticles, 10 nm

DNG-L007 10 mL
EUR 845

DiagNano Red Fluorescent Silica Nanoparticles, 30 nm

DNG-L009 10 mL
EUR 725

DiagNano Red Fluorescent Silica Nanoparticles, 50 nm

DNG-L010 10 mL
EUR 679

DiagNano Red Fluorescent Silica Nanoparticles, 70 nm

DNG-L012 10 mL
EUR 611

DiagNano Red Fluorescent Silica Nanoparticles, 100 nm

DNG-L016 10 mL
EUR 611

DiagNano Red Fluorescent Silica Nanoparticles, 200 nm

DNG-L017 10 mL
EUR 611

DiagNano Red Fluorescent Silica Particles, 300 nm

DNG-L020 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 500 nm

DNG-L025 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 1 μm

DNG-L026 10 mL
EUR 632

DiagNano Red Fluorescent Silica Particles, 1.5 μm

DNG-L029 10 mL
EUR 632

DiagNano Red Fluorescent Silica Particles, 3 μm

DNG-L032 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 5 μm

DNG-L033 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 10 μm

DNG-L036 10 mL
EUR 637

DiagNano Red Fluorescent Silica Particles, 15 μm

DNG-L038 10 mL
EUR 637

DiagNano Red Fluorescent Silica Particles, 20 μm

DNG-L039 10 mL
EUR 637

Human Mesenchymal Stem Cells-Red Fluorescent-Labeled

SC00A3 500000
EUR 962

Absolute Mag Magnetic Particles, Fluorescent Nile Red

WHM-S134 2 mL
EUR 627

Monoclonal Anti-Red Fluorescent Proteins/Tag (RFP-tag; Discosoma sea anemomone) IgG

RFP12-M 100 ug
EUR 482

RFP Expressing Human Gastric Carcinoma N87 Cells

TR02-RFP 500,000 Cells
EUR 1354

RFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-RFP 500,000 Cells
EUR 1354

Recombinant (E. coli) Red Fluorescent Proteins (RFP/dsRed) protein for ELISA or Standards (>98%)

RFP15-R 25 ug
EUR 250

RFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-RFP 500,000 Cells
EUR 1354

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 30 nm

DNG-L042 10 mL
EUR 819

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 50 nm

DNG-L043 10 mL
EUR 793

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 70 nm

DNG-L045 10 mL
EUR 788

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 100 nm

DNG-L048 10 mL
EUR 684

DiagNano Red Fluorescent, Amine Silica Particles, 200 nm

DNG-L049 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 300 nm

DNG-L052 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 500 nm

DNG-L057 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 1 μm

DNG-L058 10 mL
EUR 684

DiagNano Red Fluorescent, Amine Silica Particles, 1.5 μm

DNG-L061 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 3 μm

DNG-L065 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 4 μm

DNG-L068 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 5 μm

DNG-L069 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 10 μm

DNG-L074 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 15 μm

DNG-L077 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 20 μm

DNG-L078 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 30 nm

DNG-L082 10 mL
EUR 793

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 50 nm

DNG-L083 10 mL
EUR 767

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 70 nm

DNG-L085 10 mL
EUR 762

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 100 nm

DNG-L088 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 200 nm

DNG-L089 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 300 nm

DNG-L092 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 500 nm

DNG-L097 10 mL
EUR 684

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1 μm

DNG-L098 10 mL
EUR 684

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1.5 μm

DNG-L101 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 3 μm

DNG-L105 10 mL
EUR 684

DiagNano Red Fluorescent, Carboxyl Silica Particles, 4 μm

DNG-L108 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 5 μm

DNG-L109 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 10 μm

DNG-L114 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 15 μm

DNG-L117 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 20 μm

DNG-L118 10 mL
EUR 679

Absolute Mag Streptavidin Magnetic Particles, Fluorescent Nile Red

WHM-S119 2 mL
EUR 736

Absolute Mag Biotin Magnetic Particles, Fluorescent Nile Red

WHM-S127 5 mL
EUR 788

Absolute Mag Amine Magnetic Particles, Fluorescent Nile Red

WHM-S138 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Ferromagnetic, Fluorescent Nile Red

WHM-S011 2 mL
EUR 621

Absolute Mag Protein G Magnetic Polystyrene particles, Fluorescent Red, 2 µm

WHM-M079 1 mL
EUR 788

Fluorescent Protein Antibody Set

K817-6-100
EUR 1240

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.04-0.06 µm

DNM-L008 2 mL
EUR 657

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.1-0.3 µm

DNM-L009 2 mL
EUR 657

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L010 2 mL
EUR 657

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 1.7-2.2 µm

DNM-L036 2 mL
EUR 663

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 2.5-3.4 µm

DNM-L037 2 mL
EUR 729

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 3.5-4.5 µm

DNM-L038 2 mL
EUR 729

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 6.0-7.9 µm

DNM-L039 2 mL
EUR 729

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 8.0-9.9 µm

DNM-L040 2 mL
EUR 840

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 10.0-14.0 µm

DNM-L041 2 mL
EUR 823

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 15.0-19.0 µm

DNM-L042 2 mL
EUR 840

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.04-0.08 µm

DNM-L070 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.09-0.3 µm

DNM-L071 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.4-0.6 µm

DNM-L072 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L073 2 mL
EUR 657

Absolute Mag Amine Magnetic Dextran Nanoparticles, Fluorescent Red, 20 nm

WHM-C156 5 mL
EUR 789

Absolute Mag Plain Magnetic Dextran Particles, Fluorescent Red, 100 nm

WHM-M100 5 mL
EUR 1027

Absolute Mag Amine Magnetic Dextran Particles, Fluorescent Red, 100 nm

WHM-M101 5 mL
EUR 1027

RED antibody

22518-100ul 100ul
EUR 390

RED Antibody

20-abx249463
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

RED Antibody

DF4352 200ul
EUR 304
Description: RED Antibody detects endogenous levels of total RED.

RED Antibody

ABD4352 100 ug
EUR 438

RED antibody

70R-13184 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal RED antibody

Fluorescent

FPAK-3058--9K 9X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

DiagPoly Avidin Coated Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L096 5 mL
EUR 663

DiagPoly Biotin Coated Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L103 5 mL
EUR 663

DiagPoly Biotin Coated Fluorescent Polystyrene Particles, Nile Red, 7.0-7.9 µm

DNM-L104 5 mL
EUR 829

Absolute Mag Amine Magnetic Nanoparticles, Dextran Coated, Fluorescent Red, 100 nm

WHF-G117 5 mL
EUR 812

Absolute Mag Plain Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M097 5 mL
EUR 1027

Absolute Mag Amine Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M098 5 mL
EUR 1027

Absolute Mag Streptavidin Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M099 1 mL
EUR 835

Absolute Mag Amine Magnetic Particles, Fluorescent Nile Red, PEG Spacer Arm

WHM-S139 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.2-0.39 μm

WHM-S140 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.4-0.69 μm

WHM-S142 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.7-0.9 μm

WHM-S144 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 1.0-1.4 μm

WHM-S146 2 mL
EUR 585

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 4.0-4.9 μm

WHM-S156 2 mL
EUR 658

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 8.0-9.9 μm

WHM-S162 2 mL
EUR 694

Green Fluorescent Protein

MO22184 100 ul
EUR 409

Green Fluorescent Protein

CH22124 100 ul of 1 mg/ml
EUR 435

Cyan Fluorescent Protein

30R-2808 100 ug
EUR 527
Description: Purified recombinant Human Cyan Fluorescent Protein

dsRed Fluorescent protein

30R-2809 100 ug
EUR 491
Description: Purified recombinant Human dsRed Fluorescent protein

Fluorescent Protein Set

K816-6-100
EUR 1240

mCherry Fluorescent Protein

4993-100
EUR 408

mCherry Fluorescent Protein

4993-1000
EUR 2621

mCherry Fluorescent Protein

4993-5000
EUR 7646

Cyan Fluorescent Protein

4996-100
EUR 376

Cyan Fluorescent Protein

4996-1000
EUR 2388

Cyan Fluorescent Protein

4996-5000
EUR 6520

dsRed Fluorescent Protein

4997-100
EUR 408

dsRed Fluorescent Protein

4997-1000
EUR 2507

dsRed Fluorescent Protein

4997-5000
EUR 7189

Yellow Fluorescent Protein

4998-100
EUR 392

Yellow Fluorescent Protein

4998-1000
EUR 2513

Yellow Fluorescent Protein

4998-5000
EUR 6846

Green Fluorescent Protein (GFP) Antibody

20-abx118007
  • EUR 551.00
  • EUR 481.00
  • 100 ul
  • 50 ul

Green Fluorescent Protein (GFP) Antibody

abx034623-400ul 400 ul
EUR 523

Green Fluorescent Protein (GFP) Antibody

abx034623-80l 80 µl
EUR 286

Green Fluorescent Protein (GFP) Antibody

abx034624-100ul 100 ul
EUR 523

Green Fluorescent Protein (GFP) Antibody

abx037713-100ug 100 ug
EUR 391

Green Fluorescent Protein (GFP) Antibody

abx037714-100ug 100 ug
EUR 391

Green Fluorescent Protein (GFP) Antibody

20-abx132159
  • EUR 300.00
  • EUR 133.00
  • EUR 801.00
  • EUR 425.00
  • EUR 258.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

20-abx134154
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134155
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134156
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134157
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

abx140045-01mg 0.1 mg
EUR 384

Green Fluorescent Protein (GFP) Antibody

20-abx137422
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Green Fluorescent Protein (GFP) Antibody

20-abx005584
  • EUR 314.00
  • EUR 411.00
  • EUR 258.00
  • 100 ul
  • 200 ul
  • 50 ul

Green Fluorescent Protein (GFP) Antibody

20-abx005585
  • EUR 314.00
  • EUR 411.00
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody

abx015749-100ul 100 ul
EUR 411

Green Fluorescent Protein (GFP) Antibody

abx019093-100ug 100 ug
EUR 321

Green Fluorescent Protein (GFP) Antibody

abx019094-100ug 100 ug
EUR 321

Green Fluorescent Protein (GFP) Antibody

abx019099-100ug 100 ug
EUR 342

Green Fluorescent Protein (GFP) Antibody

20-abx020016
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Blue Fluorescent Protein (BFP) Antibody

abx412394-50ug 50 ug
EUR 495

Cyan Fluorescent Protein (CFP) Antibody

abx412395-50ug 50 ug
EUR 495

Green Fluorescent Protein (GFP) Antibody

20-abx210090
  • EUR 328.00
  • EUR 272.00
  • 100 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

20-abx159519
  • EUR 272.00
  • EUR 230.00
  • 100 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

abx233433-100ug 100 ug
EUR 481

Green Fluorescent Protein (GFP) Antibody

abx233434-100ug 100 ug
EUR 481

Green Fluorescent Protein (GFP) Antibody

abx233435-100ug 100 ug
EUR 481

Green Fluorescent Protein (GFP) Antibody

20-abx210351
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

PD1 ELISA Matched Antibody Pair (Risk Free)

RFP-0003 1 Set
EUR 626.75
Description: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications

Utilizing stagRFP ability to partner with some fret partners, we developed a new multiplex method to check the meeting of the three kinase signaling activities simultaneously in a single living cell, providing evidence for the role of Src family kinases in regulating growth factor-induced Akt and ERK activity.