Enhancer activates gene transcription in spatial and temporal patterns by interacting with the promoter of the gene. These elements usually Being distal targeting their promoters, which they must interact selectively. Additional elements can contribute to the enhancer-promoter specificity, including remote control sequences within enhancer elements, the withdrawal of the elements near the promoter, and the insulator / boundary element disturbing off-target interactions.

However, some elements have been mapped, and as a result, the mechanism which these elements interact remain poorly understood. One obstacle is the method of their studies, the reporter transgenes where enhancer positioned adjacent to a heterologous promoter, which may avoid a control mechanism enhancer-promoter specificity and long-term interaction. Here, we report a dual reporter system optimized transgenes in Drosophila melanogaster that allows simultaneous comparison of enhancing the ability to activate proximal and distal fluorescent reporter gene.

Testing a panel of fluorescent transgene in vivo, we found the combination of two proteins that allow simultaneous measurement with minimal interference detection. We note the differences between the four enhancer tested their ability to regulate reporter transgenes placed distally. These results indicate that the enhancers differ in their need for interaction promoter and raises important practical consideration when studying the function enhancer.

Red Light/Green Light, a Dual Fluorescent Protein Reporter System To Study Enhancer-Promoter Specificity in Drosophila.
Red Light/Green Light, a Dual Fluorescent Protein Reporter System To Study Enhancer-Promoter Specificity in Drosophila.

Fluorescent Protein Expression and Characterization of Far-red light from Pink-pigmented Network of Porites lobata.

Anthozoan member family display green fluorescent protein (GFP) diversity of the photo-physical properties that can be associated with normal tissue and damaged coral. Poritid coral species often show local pink pigmentation in diseased or damaged tissue. spectral analysis and our histological lesions Porites lobata pink-pigmented shows co-localization of a bright red fluorescence with amoebocytes allegedly concentrating on the epidermis, demonstrating the innate immune response is activated. Here we report the cloning, expression, and characterization of new red fluorescent protein (plobRFP) of pink-pigmented tissue associated with lesions on Porites lobata.

In vitro, recombinant plobRFP indicate different signals red emission of 614 nm (excitation maximum: 578 nm), making plobRFP red-shifted fluorescent protein isolated from a naturally furthest scleractinian coral. Recombinant proteins have higher molar extinction coefficient (84,000 M-1cm-1) and quantum results (0.74), it is important to negotiate brightness plobRFP. sequence analysis showed different brightness and redshift marked perhaps the inherent features of this plobRFP chromophore conformation.

While plobRFP to show a tendency to aggregate, high pH stability, photostability and spectral properties make it a candidate for cell imaging applications and the potential for optimized engineering template RFP. PlobRFP association with the immune response may furthers its potential use as a diagnostic and molecular biomarkers to monitor the health of coral visual. Self-associate split fluorescent protein (FP) FP is divided into two fragments which spontaneously associate to form a functional FP.

DiagNano Red Fluorescent Silica Nanoparticles, 10 nm

DNG-L007 10 mL
EUR 1014

DiagNano Red Fluorescent Silica Nanoparticles, 30 nm

DNG-L009 10 mL
EUR 870

DiagNano Red Fluorescent Silica Nanoparticles, 50 nm

DNG-L010 10 mL
EUR 814.8

DiagNano Red Fluorescent Silica Nanoparticles, 70 nm

DNG-L012 10 mL
EUR 733.2

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 100 nm

DNG-L048 10 mL
EUR 820.8

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 30 nm

DNG-L042 10 mL
EUR 982.8

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 50 nm

DNG-L043 10 mL
EUR 951.6

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 70 nm

DNG-L045 10 mL
EUR 945.6

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 100 nm

DNG-L088 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 30 nm

DNG-L082 10 mL
EUR 951.6

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 50 nm

DNG-L083 10 mL
EUR 920.4

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 70 nm

DNG-L085 10 mL
EUR 914.4

DiagNano Green Fluorescent Silica Nanoparticles, 100 nm

DNG-L015 10 mL
EUR 752.4

DiagNano Blue Fluorescent Silica Nanoparticles, 70 nm

DNG-L014 10 mL
EUR 801.6

DiagNano Green Fluorescent Silica Nanoparticles, 30 nm

DNG-L008 10 mL
EUR 870

DiagNano Green Fluorescent Silica Nanoparticles, 50 nm

DNG-L011 10 mL
EUR 820.8

DiagNano Green Fluorescent Silica Nanoparticles, 70 nm

DNG-L013 10 mL
EUR 858

DiagNano Green Fluorescent, Amine Silica Nanoparticles, 100 nm

DNG-L047 10 mL
EUR 814.8

DiagNano Green Fluorescent, Carboxyl Silica Nanoparticles, 100 nm

DNG-L087 10 mL
EUR 820.8

DiagNano Green Fluorescent, Amine Silica Nanoparticles, 30 nm

DNG-L041 10 mL
EUR 1008

DiagNano Green Fluorescent, Amine Silica Nanoparticles, 50 nm

DNG-L044 10 mL
EUR 945.6

DiagNano Green Fluorescent, Amine Silica Nanoparticles, 70 nm

DNG-L046 10 mL
EUR 945.6

DiagNano Green Fluorescent, Carboxyl Silica Nanoparticles, 30 nm

DNG-L081 10 mL
EUR 951.6

DiagNano Green Fluorescent, Carboxyl Silica Nanoparticles, 50 nm

DNG-L084 10 mL
EUR 920.4

DiagNano Green Fluorescent, Carboxyl Silica Nanoparticles, 70 nm

DNG-L086 10 mL
EUR 914.4

DiagNano Red Coloured Silica Nanoparticles, 100 nm

DNG-L122 10 mL
EUR 733.2

DiagNano Silica Nanoparticles, 100 nm

DNG-B005 10 mL
EUR 670.8

DiagNano Silica Nanoparticles, 10 nm

DNG-B001 10 mL
EUR 852

DiagNano Silica Nanoparticles, 30 nm

DNG-B002 10 mL
EUR 826.8

DiagNano Silica Nanoparticles, 50 nm

DNG-B003 10 mL
EUR 721.2

DiagNano Silica Nanoparticles, 70 nm

DNG-B004 10 mL
EUR 670.8

DiagNano TMS Silica Nanoparticles, 100 nm

DNG-F010 250 mg
EUR 764.4

DiagNano Amine Silica Nanoparticles, 100 nm

DNG-F035 10 mL
EUR 721.2

DiagNano TMS Silica Nanoparticles, 50 nm

DNG-F009 250 mg
EUR 838.8

DiagNano Acrylate Silica Nanoparticles, 100 nm

DNG-F004 10 mL
EUR 739.2

DiagNano Carboxyl Silica Nanoparticles, 100 nm

DNG-F050 10 mL
EUR 721.2

DiagNano Amine Silica Nanoparticles, 30 nm

DNG-F032 10 mL
EUR 908.4

DiagNano Amine Silica Nanoparticles, 50 nm

DNG-F033 10 mL
EUR 795.6

DiagNano Amine Silica Nanoparticles, 70 nm

DNG-F034 10 mL
EUR 764.4

DiagNano Acrylate Silica Nanoparticles, 30 nm

DNG-F001 10 mL
EUR 926.4

DiagNano Acrylate Silica Nanoparticles, 50 nm

DNG-F002 10 mL
EUR 814.8

DiagNano Acrylate Silica Nanoparticles, 70 nm

DNG-F003 10 mL
EUR 776.4

DiagNano Carboxyl Silica Nanoparticles, 30 nm

DNG-F047 10 mL
EUR 883.2

DiagNano Carboxyl Silica Nanoparticles, 50 nm

DNG-F048 10 mL
EUR 820.8

DiagNano Carboxyl Silica Nanoparticles, 70 nm

DNG-F049 10 mL
EUR 795.6

DiagNano Streptavidin Silica Nanoparticles, 100 nm

DNG-C011 1 mL
EUR 764.4

DiagNano Blue Coloured Silica Nanoparticles, 100 nm

DNG-L121 10 mL
EUR 727.2

DiagNano™ PEI Mesoporous Silica Nanoparticles, 100 nm

WHM-23DN45 5 mL
EUR 1600

DiagNano™ Plain Mesoporous Silica Nanoparticles, 100 nm

WHM-23DN41 5 mL
EUR 1160

DiagNano™ Amine Mesoporous Silica Nanoparticles, 100 nm

WHM-23DN43 5 mL
EUR 1220

DiagNano™ Thiol Mesoporous Silica Nanoparticles, 100 nm

WHM-23DN47 5 mL
EUR 1600

DiagNano™ S-S Mesoporous Silica Nanoparticles, 100 nm

WHM-23DN46 5 mL
EUR 1600

DiagNano™ PEI Mesoporous Silica Nanoparticles, 50 nm

WHM-23DN27 5 mL
EUR 1600

DiagNano Silica-Coated Gold Nanoparticles, 10 nm

CG-10 10mL
EUR 862.8

DiagNano Silica-Coated Gold Nanoparticles, 20 nm

CG-20 10mL
EUR 862.8

DiagNano Silica-Coated Gold Nanoparticles, 5 nm

CG-5 10mL
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DiagNano™ Carboxyl Mesoporous Silica Nanoparticles, 100 nm

WHM-23DN42 5 mL
EUR 1220

DiagNano™ Plain Mesoporous Silica Nanoparticles, 50 nm

WHM-23DN23 5 mL
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DiagNano™ Amine Mesoporous Silica Nanoparticles, 50 nm

WHM-23DN25 5 mL
EUR 1220

DiagNano™ Thiol Mesoporous Silica Nanoparticles, 50 nm

WHM-23DN29 5 mL
EUR 1600

DiagNano™ S-S Mesoporous Silica Nanoparticles, 50 nm

WHM-23DN28 5 mL
EUR 1600

DiagNano Silica-Coated Gold Nanoparticles, 10 nm, 100 OD

CG-10-100 0.25mL
EUR 926.4

DiagNano Silica-Coated Gold Nanoparticles, 20 nm, 100 OD

CG-20-100 0.25mL
EUR 926.4

DiagNano Silica-Coated Gold Nanoparticles, 5 nm, 100 OD

CG-5-100 0.25mL
EUR 1088.4

DiagNano™ Carboxyl Mesoporous Silica Nanoparticles, 50 nm

WHM-23DN24 5 mL
EUR 1220

DiagNano™ PEG Carboxyl Mesoporous Silica Nanoparticles, 100 nm

WHM-23DN44 5 mL
EUR 1600

DiagNano Red Fluorescent Silica Particles, 300 nm

DNG-L020 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 500 nm

DNG-L025 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 1 μm

DNG-L026 10 mL
EUR 758.4

DiagNano Red Fluorescent Silica Particles, 1.5 μm

DNG-L029 10 mL
EUR 758.4

DiagNano Red Fluorescent Silica Particles, 3 μm

DNG-L032 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 5 μm

DNG-L033 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 10 μm

DNG-L036 10 mL
EUR 764.4

DiagNano Red Fluorescent Silica Particles, 15 μm

DNG-L038 10 mL
EUR 764.4

DiagNano Red Fluorescent Silica Particles, 20 μm

DNG-L039 10 mL
EUR 764.4

DiagNano Dense Silica Coated Upconverting Nanoparticles, 804 nm

DNL-S005 10 mg
EUR 1076.4

DiagNano™ PEG Carboxyl Mesoporous Silica Nanoparticles, 50 nm

WHM-23DN26 5 mL
EUR 1600

DiagNano™ Dense Silica Upconverting Nanoparticles, 100 nm, 804 nm

DNL-C020 10 mg
EUR 1080

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 10 nm

PG-10 10mL
EUR 862.8

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm

PG-20 10mL
EUR 862.8

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PG-5 10mL
EUR 862.8

DiagNano™ Dense Silica-COOH Upconverting Nanoparticles, 545 nm

DNL-H006 10 mg
EUR 1300

DiagNano™ Dense Silica-COOH Upconverting Nanoparticles, 804 nm

DNL-H008 10 mg
EUR 1300

DiagNano™ Dense Silica Coated Upconverting Nanoparticles, 475 nm

DNL-S002 10 mg
EUR 1780

DiagNano™ Dense Silica Coated Upconverting Nanoparticles, 660 nm

DNL-S004 10 mg
EUR 1780

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 10 nm, 100 OD

PG-10-100 0.25mL
EUR 1101.6

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm, 100 OD

PG-20-100 0.25mL
EUR 1101.6

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 5 nm, 100 OD

PG-5-100 0.25mL
EUR 1275.6

DiagNano Dense Silica Coated Upconverting Nanoparticles, 365 nm/475 nm

DNL-S001 10 mg
EUR 1076.4

DiagNano Dense Silica Coated Upconverting Nanoparticles, 545 nm/660 nm

DNL-S003 10 mg
EUR 1076.4

DiagNano™ Dense Silica Upconverting Nanoparticles, 100 nm, 365 nm/475 nm

DNL-C018 10 mg
EUR 1080

DiagNano™ Dense Silica Upconverting Nanoparticles, 100 nm, 545 nm/660 nm

DNL-C019 10 mg
EUR 1080

DiagNano™ Mesoporous Silica Coated Upconverting Nanoparticles, 365 nm

DNL-S006 10 mg
EUR 1780

DiagNano™ Mesoporous Silica Coated Upconverting Nanoparticles, 475 nm

DNL-S007 10 mg
EUR 1780

DiagNano™ Mesoporous Silica Coated Upconverting Nanoparticles, 545 nm

DNL-S008 10 mg
EUR 1780

DiagNano™ Mesoporous Silica Coated Upconverting Nanoparticles, 660 nm

DNL-S009 10 mg
EUR 1780

DiagNano™ Mesoporous Silica Coated Upconverting Nanoparticles, 804 nm

DNL-S010 10 mg
EUR 1780

DiagNano™ Dense Silica-COOH Upconverting Nanoparticles, 365 nm/475 nm

DNL-H005 10 mg
EUR 1300

DiagNano™ Dense Silica-COOH Upconverting Nanoparticles, 545 nm/660 nm

DNL-H007 10 mg
EUR 1300

DiagNano Red Fluorescent, Amine Silica Particles, 200 nm

DNG-L049 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 300 nm

DNG-L052 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 500 nm

DNG-L057 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 1 μm

DNG-L058 10 mL
EUR 820.8

DiagNano Red Fluorescent, Amine Silica Particles, 1.5 μm

DNG-L061 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 3 μm

DNG-L065 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 4 μm

DNG-L068 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 5 μm

DNG-L069 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 10 μm

DNG-L074 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 15 μm

DNG-L077 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 20 μm

DNG-L078 10 mL
EUR 814.8

DiagPoly™ Plain Fluorescent Polystyrene Nanoparticles, Flash Red, 0.05 µm

DNBF-P02 1 mL
EUR 810

DiagPoly™ Plain Fluorescent Polystyrene Nanoparticles, Flash Red, 0.2 µm

DNBF-P06 1 mL
EUR 810

DiagNano™ PEI Monodisperse Mesoporous Silica Nanoparticles, Hollow, 150 nm

WHM-23DN06 5 mL
EUR 2100

DiagNano™ PEI Monodisperse Mesoporous Silica Nanoparticles, Hollow, 350 nm

WHM-23DN13 5 mL
EUR 2100

DiagNano™ S-S Monodisperse Mesoporous Silica Nanoparticles, Hollow, 150 nm

WHM-23DN07 5 mL
EUR 2100

DiagNano™ S-S Monodisperse Mesoporous Silica Nanoparticles, Hollow, 350 nm

WHM-23DN14 5 mL
EUR 2100

DiagNano Red Fluorescent, Carboxyl Silica Particles, 200 nm

DNG-L089 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 300 nm

DNG-L092 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 500 nm

DNG-L097 10 mL
EUR 820.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1 μm

DNG-L098 10 mL
EUR 820.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1.5 μm

DNG-L101 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 3 μm

DNG-L105 10 mL
EUR 820.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 4 μm

DNG-L108 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 5 μm

DNG-L109 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 10 μm

DNG-L114 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 15 μm

DNG-L117 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 20 μm

DNG-L118 10 mL
EUR 814.8

DiagNano™ Plain Monodisperse Mesoporous Silica Nanoparticles, Hollow, 150 nm

WHM-23DN02 5 mL
EUR 1460

DiagNano™ Amine Monodisperse Mesoporous Silica Nanoparticles, Hollow, 150 nm

WHM-23DN04 5 mL
EUR 1600

DiagNano™ Thiol Monodisperse Mesoporous Silica Nanoparticles, Hollow, 150 nm

WHM-23DN08 5 mL
EUR 2100

DiagNano™ Plain Monodisperse Mesoporous Silica Nanoparticles, Hollow, 350 nm

WHM-23DN09 5 mL
EUR 1460

DiagNano™ Amine Monodisperse Mesoporous Silica Nanoparticles, Hollow, 350 nm

WHM-23DN11 5 mL
EUR 1600

DiagNano™ Thiol Monodisperse Mesoporous Silica Nanoparticles, Hollow, 350 nm

WHM-23DN15 5 mL
EUR 2100

DiagNano™ PEI Monodisperse Mesoporous Silica Nanoparticles, Dendritic, 200 nm

WHM-23DN20 5 mL
EUR 2100

DiagPoly™ Carboxyl Fluorescent Polystyrene Nanoparticles, Flash Red, 0.05 µm

DNBF-C02 1 mL
EUR 810

DiagPoly™ Carboxyl Fluorescent Polystyrene Nanoparticles, Flash Red, 0.2 µm

DNBF-C06 1 mL
EUR 810

DiagNano™ Carboxyl Monodisperse Mesoporous Silica Nanoparticles, Hollow, 150 nm

WHM-23DN03 5 mL
EUR 1600

DiagNano™ Carboxyl Monodisperse Mesoporous Silica Nanoparticles, Hollow, 350 nm

WHM-23DN10 5 mL
EUR 1600

DiagNano™ Plain Monodisperse Mesoporous Silica Nanoparticles, Dendritic, 200 nm

WHM-23DN16 5 mL
EUR 1460

DiagNano™ Amine Monodisperse Mesoporous Silica Nanoparticles, Dendritic, 200 nm

WHM-23DN18 5 mL
EUR 1600

DiagNano™ Thiol Monodisperse Mesoporous Silica Nanoparticles, Dendritic, 200 nm

WHM-23DN22 5 mL
EUR 2100

DiagNano™ S-S Monodisperse Mesoporous Silica Nanoparticles, Dendritic, 200 nm

WHM-23DN21 5 mL
EUR 2100

DiagNano™ Carboxyl Monodisperse Mesoporous Silica Nanoparticles, Dendritic, 200 nm

WHM-23DN17 5 mL
EUR 1600

DiagNano™ PEG Monodisperse Carboxyl Mesoporous Silica Nanoparticles, Hollow, 150 nm

WHM-23DN05 5 mL
EUR 2100

DiagNano™ PEG Monodisperse Carboxyl Mesoporous Silica Nanoparticles, Hollow, 350 nm

WHM-23DN12 5 mL
EUR 2100

DiagNano™ Red Fluorescent, Streptavidin Silica Particles, 100 nm

DNG-L268 1 mL
EUR 822.5

DiagNano™ Red Fluorescent, Streptavidin Silica Particles, 300 nm

DNG-L269 1 mL
EUR 822.5

DiagNano™ Red Fluorescent, Streptavidin Silica Particles, 500 nm

DNG-L270 1 mL
EUR 822.5

DiagNano™ Red Fluorescent, Streptavidin Silica Particles, 3 µm

DNG-L271 1 mL
EUR 822.5

DiagNano™ Red Fluorescent, Streptavidin Silica Particles, 5 µm

DNG-L272 1 mL
EUR 822.5

DiagNano™ PEG Carboxyl Monodisperse Mesoporous Silica Nanoparticles, Dendritic, 200 nm

WHM-23DN19 5 mL
EUR 2100

DiagPoly™ Plain Fluorescent Poly(Methyl Methacrylate) PMMA Nanoparticles, Red, 25 nm

DNG-P009 10 mL
EUR 785

DiagPoly™ Amine Fluorescent Poly(Methyl Methacrylate) PMMA Nanoparticles, Red, 25 nm

DNG-P012 5 mL
EUR 755

DiagPoly™ Carboxyl Fluorescent Poly(Methyl Methacrylate) PMMA Nanoparticles, Red, 25 nm

DNG-P013 5 mL
EUR 725

Absolute Mag Amine Magnetic Dextran Nanoparticles, Fluorescent Red, 20 nm

WHM-C156 5 mL
EUR 946.8

DiagPoly™ Amine Fluorescent Fluorescent Poly(Methyl Methacrylate) PMMA Nanoparticles, Green, 25 nm

DNG-P011 5 mL
EUR 755

Absolute Mag Amine Magnetic Nanoparticles, Dextran Coated, Fluorescent Red, 100 nm

WHF-G117 5 mL
EUR 974.4

DiagNano Blue Fluorescent Silica Particles, 200 nm

DNG-L018 10 mL
EUR 801.6

They have been widely used to label proteins, protein scaffolds assembly and detecting cell-cell contacts. Recent developments have expanded palette associating themselves outside the FP split the original split GFP1-10 / 11. However, the new one has suffered from suboptimal fluorescence signal after complementation.