Enhancer activates gene transcription in spatial and temporal patterns by interacting with the promoter of the gene. These elements usually Being distal targeting their promoters, which they must interact selectively. Additional elements can contribute to the enhancer-promoter specificity, including remote control sequences within enhancer elements, the withdrawal of the elements near the promoter, and the insulator / boundary element disturbing off-target interactions.
However, some elements have been mapped, and as a result, the mechanism which these elements interact remain poorly understood. One obstacle is the method of their studies, the reporter transgenes where enhancer positioned adjacent to a heterologous promoter, which may avoid a control mechanism enhancer-promoter specificity and long-term interaction. Here, we report a dual reporter system optimized transgenes in Drosophila melanogaster that allows simultaneous comparison of enhancing the ability to activate proximal and distal fluorescent reporter gene.
Testing a panel of fluorescent transgene in vivo, we found the combination of two proteins that allow simultaneous measurement with minimal interference detection. We note the differences between the four enhancer tested their ability to regulate reporter transgenes placed distally. These results indicate that the enhancers differ in their need for interaction promoter and raises important practical consideration when studying the function enhancer.

Fluorescent Protein Expression and Characterization of Far-red light from Pink-pigmented Network of Porites lobata.
Anthozoan member family display green fluorescent protein (GFP) diversity of the photo-physical properties that can be associated with normal tissue and damaged coral. Poritid coral species often show local pink pigmentation in diseased or damaged tissue. spectral analysis and our histological lesions Porites lobata pink-pigmented shows co-localization of a bright red fluorescence with amoebocytes allegedly concentrating on the epidermis, demonstrating the innate immune response is activated. Here we report the cloning, expression, and characterization of new red fluorescent protein (plobRFP) of pink-pigmented tissue associated with lesions on Porites lobata.
In vitro, recombinant plobRFP indicate different signals red emission of 614 nm (excitation maximum: 578 nm), making plobRFP red-shifted fluorescent protein isolated from a naturally furthest scleractinian coral. Recombinant proteins have higher molar extinction coefficient (84,000 M-1cm-1) and quantum results (0.74), it is important to negotiate brightness plobRFP. sequence analysis showed different brightness and redshift marked perhaps the inherent features of this plobRFP chromophore conformation.
While plobRFP to show a tendency to aggregate, high pH stability, photostability and spectral properties make it a candidate for cell imaging applications and the potential for optimized engineering template RFP. PlobRFP association with the immune response may furthers its potential use as a diagnostic and molecular biomarkers to monitor the health of coral visual. Self-associate split fluorescent protein (FP) FP is divided into two fragments which spontaneously associate to form a functional FP.
DiagNano Red Fluorescent Silica Nanoparticles, 10 nm |
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DNG-L007 | Creative Diagnostics | 10 mL | EUR 1014 |
DiagNano Red Fluorescent Silica Nanoparticles, 30 nm |
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DNG-L009 | Creative Diagnostics | 10 mL | EUR 870 |
DiagNano Red Fluorescent Silica Nanoparticles, 50 nm |
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DNG-L010 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Red Fluorescent Silica Nanoparticles, 70 nm |
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DNG-L012 | Creative Diagnostics | 10 mL | EUR 733.2 |
DiagNano Red Fluorescent, Amine Silica Nanoparticles, 100 nm |
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DNG-L048 | Creative Diagnostics | 10 mL | EUR 820.8 |
DiagNano Red Fluorescent, Amine Silica Nanoparticles, 30 nm |
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DNG-L042 | Creative Diagnostics | 10 mL | EUR 982.8 |
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DiagNano Red Fluorescent, Amine Silica Nanoparticles, 70 nm |
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DNG-L088 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 30 nm |
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DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 50 nm |
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DNG-L083 | Creative Diagnostics | 10 mL | EUR 920.4 |
DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 70 nm |
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DiagNano Green Fluorescent Silica Nanoparticles, 30 nm |
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DiagNano Green Fluorescent, Carboxyl Silica Nanoparticles, 70 nm |
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DNG-B002 | Creative Diagnostics | 10 mL | EUR 826.8 |
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DNG-F010 | Creative Diagnostics | 250 mg | EUR 764.4 |
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DNG-F035 | Creative Diagnostics | 10 mL | EUR 721.2 |
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DNG-F009 | Creative Diagnostics | 250 mg | EUR 838.8 |
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DNG-F004 | Creative Diagnostics | 10 mL | EUR 739.2 |
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DNG-F032 | Creative Diagnostics | 10 mL | EUR 908.4 |
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DNG-F033 | Creative Diagnostics | 10 mL | EUR 795.6 |
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DNG-F034 | Creative Diagnostics | 10 mL | EUR 764.4 |
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DNG-F047 | Creative Diagnostics | 10 mL | EUR 883.2 |
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DNG-F048 | Creative Diagnostics | 10 mL | EUR 820.8 |
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CG-10 | Creative Diagnostics | 10mL | EUR 862.8 |
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CG-10-100 | Creative Diagnostics | 0.25mL | EUR 926.4 |
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DNL-S005 | Creative Diagnostics | 10 mg | EUR 1076.4 |
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PG-10 | Creative Diagnostics | 10mL | EUR 862.8 |
DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm |
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PG-20 | Creative Diagnostics | 10mL | EUR 862.8 |
DiagNano Silica-Coated PEGylated Gold Nanoparticles, 5 nm |
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PG-5 | Creative Diagnostics | 10mL | EUR 862.8 |
DiagNano Silica-Coated PEGylated Gold Nanoparticles, 10 nm, 100 OD |
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PG-10-100 | Creative Diagnostics | 0.25mL | EUR 1101.6 |
DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm, 100 OD |
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PG-20-100 | Creative Diagnostics | 0.25mL | EUR 1101.6 |
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PG-5-100 | Creative Diagnostics | 0.25mL | EUR 1275.6 |
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DNL-S001 | Creative Diagnostics | 10 mg | EUR 1076.4 |
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DNG-L026 | Creative Diagnostics | 10 mL | EUR 758.4 |
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DiagNano Red Fluorescent Silica Particles, 5 μm |
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DiagNano Red Fluorescent Silica Particles, 10 μm |
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DiagNano Red Fluorescent Silica Particles, 15 μm |
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DiagNano Red Fluorescent Silica Particles, 20 μm |
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DiagNano Red Fluorescent, Amine Silica Particles, 200 nm |
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DiagNano Red Fluorescent, Amine Silica Particles, 300 nm |
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DiagNano Red Fluorescent, Amine Silica Particles, 500 nm |
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DiagNano Red Fluorescent, Amine Silica Particles, 1 μm |
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DNG-L058 | Creative Diagnostics | 10 mL | EUR 820.8 |
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DiagNano Red Fluorescent, Amine Silica Particles, 5 μm |
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DiagNano Red Fluorescent, Amine Silica Particles, 10 μm |
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DiagNano Red Fluorescent, Amine Silica Particles, 15 μm |
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DiagNano Red Fluorescent, Amine Silica Particles, 20 μm |
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DiagNano Red Fluorescent, Carboxyl Silica Particles, 200 nm |
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DNG-L089 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Red Fluorescent, Carboxyl Silica Particles, 300 nm |
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DNG-L092 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Red Fluorescent, Carboxyl Silica Particles, 500 nm |
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DNG-L097 | Creative Diagnostics | 10 mL | EUR 820.8 |
DiagNano Red Fluorescent, Carboxyl Silica Particles, 1 μm |
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DiagNano Red Fluorescent, Carboxyl Silica Particles, 1.5 μm |
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DNG-L101 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Red Fluorescent, Carboxyl Silica Particles, 3 μm |
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DNG-L105 | Creative Diagnostics | 10 mL | EUR 820.8 |
DiagNano Red Fluorescent, Carboxyl Silica Particles, 4 μm |
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DiagNano Red Fluorescent, Carboxyl Silica Particles, 5 μm |
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DiagNano Red Fluorescent, Carboxyl Silica Particles, 10 μm |
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DNG-L114 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Red Fluorescent, Carboxyl Silica Particles, 15 μm |
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DNG-L117 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Red Fluorescent, Carboxyl Silica Particles, 20 μm |
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DiagNano Fluorophore Labeled Gold Nanoparticles, 100 nm |
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GFL-100 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 125 nm |
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GFL-125 | Creative Diagnostics | 1 mL | EUR 1263.6 |
Absolute Mag Amine Magnetic Dextran Nanoparticles, Fluorescent Red, 20 nm |
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WHM-C156 | Creative Diagnostics | 5 mL | EUR 946.8 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 10 nm |
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GFL-10 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 15 nm |
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GFL-15 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 25 nm |
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GFL-25 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 30 nm |
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GFL-30 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 35 nm |
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GFL-35 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 40 nm |
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GFL-40 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 45 nm |
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GFL-45 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 5 nm |
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GFL-5 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 50 nm |
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GFL-50 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 55 nm |
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GFL-55 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 60 nm |
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GFL-60 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 70 nm |
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GFL-70 | Creative Diagnostics | 1 mL | EUR 1263.6 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 75 nm |
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WHF-G117 | Creative Diagnostics | 5 mL | EUR 974.4 |
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DNG-L018 | Creative Diagnostics | 10 mL | EUR 801.6 |
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DiagNano Blue Fluorescent Silica Particles, 1 μm |
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DiagNano Green Fluorescent Silica Particles, 300 nm |
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DiagNano Green Fluorescent Silica Particles, 500 nm |
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DiagNano Green Fluorescent Silica Particles, 1 μm |
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DiagNano Green Fluorescent Silica Particles, 1.5 μm |
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DiagNano Green Fluorescent Silica Particles, 10 μm |
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DNL-F010 | Creative Diagnostics | 10 mg | EUR 1138.8 |
Absolute Mag Streptavidin Magnetic Dextran Nanoparticles, Dextran Coated, Fluorescent Red, 300 nm |
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WHM-G258 | Creative Diagnostics | 1 mL | EUR 1070.4 |
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GFL-10-CU | Creative Diagnostics | 1mL | EUR 1638 |
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GFL-15-CU | Creative Diagnostics | 1mL | EUR 1638 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 20 nm, Cellular Uptake |
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GFL-20-CU | Creative Diagnostics | 1mL | EUR 1638 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 25 nm, Cellular Uptake |
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GFL-25-CU | Creative Diagnostics | 1mL | EUR 1638 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 30 nm, Cellular Uptake |
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GFL-30-CU | Creative Diagnostics | 1mL | EUR 1638 |
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GFL-35-CU | Creative Diagnostics | 1mL | EUR 1638 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 40 nm, Cellular Uptake |
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GFL-40-CU | Creative Diagnostics | 1mL | EUR 1638 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 45 nm, Cellular Uptake |
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GFL-45-CU | Creative Diagnostics | 1mL | EUR 1638 |
DiagNano Fluorophore Labeled Gold Nanoparticles, 50 nm, Cellular Uptake |
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GFL-50-CU | Creative Diagnostics | 1mL | EUR 1638 |
DiagNano Blue Fluorescent, Amine Silica Particles, 200 nm |
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DNG-L050 | Creative Diagnostics | 10 mL | EUR 838.8 |
DiagNano Blue Fluorescent, Amine Silica Particles, 300 nm |
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DNG-L054 | Creative Diagnostics | 10 mL | EUR 838.8 |
DiagNano Blue Fluorescent, Amine Silica Particles, 500 nm |
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DiagNano Blue Fluorescent, Amine Silica Particles, 1 μm |
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DiagNano Blue Fluorescent, Amine Silica Particles, 3 μm |
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DiagNano Blue Fluorescent, Amine Silica Particles, 4 μm |
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DNG-L067 | Creative Diagnostics | 10 mL | EUR 838.8 |
DiagNano Blue Fluorescent, Amine Silica Particles, 5 μm |
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DiagNano Blue Fluorescent, Amine Silica Particles, 10 μm |
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DiagNano Blue Fluorescent, Amine Silica Particles, 15 μm |
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DiagNano Blue Fluorescent, Amine Silica Particles, 20 μm |
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DNG-L080 | Creative Diagnostics | 10 mL | EUR 838.8 |
DiagNano HIV p24 Conjugated Gold Nanoparticles |
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ACG-HIV05 | Creative Diagnostics | 5 mL | EUR 1376.4 |
Absolute Mag Rhodamine B Fluorescent Iron Oxide Nanoparticles, 10 nm |
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WNM-X061 | Creative Diagnostics | 2 mL | EUR 826.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 200 nm |
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DNG-L051 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 300 nm |
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DNG-L053 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 500 nm |
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DNG-L055 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 1 μm |
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DNG-L060 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 1.5 μm |
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DNG-L062 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 3 μm |
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DNG-L064 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 4 μm |
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DNG-L066 | Creative Diagnostics | 10 mL | EUR 814.8 |
DiagNano Green Fluorescent, Amine Silica Particles, 5 μm |
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DNG-L071 | Creative Diagnostics | 10 mL | EUR 814.8 |
They have been widely used to label proteins, protein scaffolds assembly and detecting cell-cell contacts. Recent developments have expanded palette associating themselves outside the FP split the original split GFP1-10 / 11. However, the new one has suffered from suboptimal fluorescence signal after complementation.