fluorescent proteins can produce reactive oxygen species (ROS) on the absorption of photons through type I and II photosensitization mechanisms. KillerRed red fluorescent protein and Supernova are phototoxic protein engineered to produce ROS and used in a variety of biological applications. However, their relative quantum results and the level of ROS production is unclear, which has limited the interpretation of their effects when used in a biological system.

We cloned and purified KillerRed, Supernova, and mCherry – red fluorescent protein linked not usually considered photosensitizer – and measuring superoxide (O2 • -) and singlet oxygen (1O2) quantum results with irradiation at 561 nm. The formation of O2 • 2-hydroxyethidium –specific product (2-OHE +) was quantified by HPLC with fluorescence detection separation. In connection with the reference photosensitizer, Rose Bengal, O2 • – quantum results (ΦO2 • -) of Supernova was determined to be 1.5 × 10-3, KillerRed is 0.97 × 10-3 and 1.2 × 10-3 mCherry , At 916.5 excitation fluence J / cm2 and suitable absorption at 561 nm, Supernova, KillerRed and mCherry made 3.81, 2.38 and 1.65 pM O2 • – / min, respectively.

Using Singlet Oxygen Sensor probe Green (SOSG), we confirmed the results of quantum 1O2 (Φ1O2) for Supernova be 22.0 × 10-3, KillerRed 7.6 × 10-3 and 5.7 × 10-3 mCherry. This photosensitization characteristics of Supernova, KillerRed and mCherry improve our understanding of the relevant fluorescent protein and to improve their use as a tool for advancing our knowledge of redox biology.

Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.
Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

Green Fluorescent protein-and Discosoma sp. Red Fluorescent Protein-Tagged Lines for Protein Marker organelle subcellular localization in Rice.

Subcellular localization of proteins is a fundamental aspect of protein function. Determine the subcellular localization is important to understand the biological function of the protein. Here, we developed a set of rice organelle marker lines, which express the organelle fluorescent marker can be used as a standard of comparison in determining the subcellular localization of the protein of interest. We build green fluorescent protein (GFP) – and / or Discosoma sp. red fluorescent protein (DsRed) -tagged organelle markers targeted to the endoplasmic reticulum (ER), mitochondria, Golgi apparatus, peroxisome, actin cytoskeleton, plastids, tonoplast, plasma membrane and nucleus, respectively.

The utility of the marker lines of rice for subcellular protein localization studies indicated by detecting OsWRKY45 local nucleus and mitochondria-related NbHxk1 in protoplasts of GFP-OsH2B and ScCOX4 line-DsRed, respectively. Using the sheath-inoculation method, followed by the live-cell imaging, we detect the co-localization of Magnaporthe oryzae PWL2: mCherry: NLS fusion with GFP marker-OsH2B core in rice epidermal cells, confirming the translocation of M. oryzae effectors into host cells PWL2 , and further demonstrates the feasibility of using line organelle markers for studying the dynamics of proteins in the cell of rice in the rice and the interactions between pathogens.

Set line organelle markers developed in this study provides a valuable resource for the study of subcellular localization of proteins in the protein fluorescent bioimaging rice.Photoconvertible have significant power and biomedical advances. However, the molecular mechanisms for light-induced processes have remained elusive because of the challenge of tracking chromophore structural dynamics during photoconversion.

Monoclonal Anti-Red Fluorescent Proteins/Tag (RFP-tag; Discosoma sea anemomone) IgG
RFP12-M 100 ug
EUR 578.4

Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody
MBS525007-005mg 0.05mg
EUR 210

Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody
MBS525007-01mg 0.1mg
EUR 255

Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody
MBS525007-02mg 0.2mg
EUR 325

Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody
MBS525007-1mg 1mg
EUR 780

Red Fluorescent Protein (RFP) Antibody
abx412396-100g 100 µg
EUR 450

Red Fluorescent Protein (RFP) Antibody
abx412396-50ug 50 ug
EUR 678

Red Fluorescent Protein (RFP) Antibody
abx240273-100l 100 µl Ask for price

Red Fluorescent Protein (RFP) Antibody
abx240273-50l 50 µl
EUR 350

Mouse Anti Green Fluorescent Protein Monoclonal Antibody
DMABT-54770MG 0.1 mg
EUR 709.8
Description: Mouse

Green Fluorescent Protein (GFP) Monoclonal Antibody
CAU30520-100ul 100ul
EUR 308.2

Green Fluorescent Protein (GFP) Monoclonal Antibody
CAU30520-200ul 200ul
EUR 384.7

Green Fluorescent Protein (GFP) Monoclonal Antibody
CAU30797-100ul 100ul
EUR 308.2

Green Fluorescent Protein (GFP) Monoclonal Antibody
CAU30797-200ul 200ul
EUR 384.7

Monoclonal Antibody to Green Fluorescent Protein (GFP)
MAD025Ge21 100ul
EUR 321

Monoclonal Antibody to Green Fluorescent Protein (GFP)
MAD025Ge22 100ul
EUR 321

Monoclonal Antibody to Green Fluorescent Protein (GFP)
MAD025Ge51 100ul
EUR 332

Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2139231-INQUIRE INQUIRE Ask for price

Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2125135-INQUIRE INQUIRE Ask for price

Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2111290-INQUIRE INQUIRE Ask for price

Anti-RFP (Red Fluorescent Protein) Antibody
GWB-3BF397 100 ug Ask for price

Red Fluorescent Protein (RFP)
MBS6508207-01mg 0.1mg
EUR 770

Red Fluorescent Protein (RFP)
MBS6508207-5x01mg 5x0.1mg
EUR 3320

PE Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2135837-INQUIRE INQUIRE Ask for price

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species)
4-MAD025Ge21
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP)

APC Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2135840-INQUIRE INQUIRE Ask for price

Cy3 Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2135843-INQUIRE INQUIRE Ask for price

HRP Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2135852-INQUIRE INQUIRE Ask for price

APC_Cy7 Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2135834-INQUIRE INQUIRE Ask for price

FITC Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2135849-INQUIRE INQUIRE Ask for price

PE-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2109806-INQUIRE INQUIRE Ask for price

PE-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2124990-INQUIRE INQUIRE Ask for price

APC-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2109808-INQUIRE INQUIRE Ask for price

Cy3-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2109810-INQUIRE INQUIRE Ask for price

HRP-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2109816-INQUIRE INQUIRE Ask for price

APC-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2124991-INQUIRE INQUIRE Ask for price

Cy3-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2124992-INQUIRE INQUIRE Ask for price

HRP-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2124995-INQUIRE INQUIRE Ask for price

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), APC
4-MAD025Ge21-APC
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with APC.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), Cy3
4-MAD025Ge21-Cy3
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with Cy3.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), HRP
4-MAD025Ge21-HRP
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with HRP.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), PE
4-MAD025Ge21-PE
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with PE.

Biotin Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2135846-INQUIRE INQUIRE Ask for price

Botin-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2109812-INQUIRE INQUIRE Ask for price

FITC-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2109814-INQUIRE INQUIRE Ask for price

FITC-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2124994-INQUIRE INQUIRE Ask for price

APC/Cy7 Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2109804-INQUIRE INQUIRE Ask for price

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), FITC
4-MAD025Ge21-FITC
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with FITC.

Biotin-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2124993-INQUIRE INQUIRE Ask for price

APC/Cy7-Linked Monoclonal Antibody to Green Fluorescent Protein (GFP)
MBS2124989-INQUIRE INQUIRE Ask for price

Red Fluorescent Protein (RFP) (AP)
MBS6497151-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (AP)
MBS6497151-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (PE)
MBS6497161-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (PE)
MBS6497161-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (APC)
MBS6497152-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (APC)
MBS6497152-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (HRP)
MBS6497155-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (HRP)
MBS6497155-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (FITC)
MBS6497154-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (FITC)
MBS6497154-5x01mL 5x0.1mL
EUR 4620

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), APC-Cy7
4-MAD025Ge21-APC-Cy7
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with APC-Cy7.

Red Fluorescent Protein (RFP) (Biotin)
MBS6497153-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (Biotin)
MBS6497153-5x01mL 5x0.1mL
EUR 4620

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), Biotinylated
4-MAD025Ge21-Biotin
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with Biotin.

RFP Tag Mouse Monoclonal Antibody (RF5R)
MBS588123-01mg 0.1mg
EUR 340

RFP Tag Mouse Monoclonal Antibody (RF5R)
MBS588123-5x01mg 5x0.1mg
EUR 1280

YFP | Yellow Fluorescent Protein (monoclonal)
AS18-4177 100 µg
EUR 331

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) IgG
GFP12-M 100 ug
EUR 578.4

Red Fluorescent Protein (RFP) (MaxLight 405)
MBS6497156-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 405)
MBS6497156-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 490)
MBS6497157-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 490)
MBS6497157-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 550)
MBS6497158-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 550)
MBS6497158-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 650)
MBS6497159-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 650)
MBS6497159-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 750)
MBS6497160-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 750)
MBS6497160-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein, Pre-adsorbed (RFP)
MBS6506872-01mg 0.1mg
EUR 715

Red Fluorescent Protein, Pre-adsorbed (RFP)
MBS6506872-5x01mg 5x0.1mg
EUR 3075

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-HRP conjugate
GFP12-HRP 0.5 ml
EUR 489.6

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-FITC conjugate
GFP12-FITC 0.5 ml
EUR 489.6

Red Fluorescent Protein (dsRed) Antibody
3994-100 each
EUR 451.2

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-Biotin conjugate
GFP12-BTN 0.5 ml
EUR 489.6

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-Alk Phos (AP) conjugate
GFP12-AP 0.5 ml
EUR 489.6

Rabbit anti Red Fluorescent Protein
MBS225348-005mg 0.05mg
EUR 460

Rabbit anti Red Fluorescent Protein
MBS225348-5x005mg 5x0.05mg
EUR 1905

RFP-Tag Mouse Monoclonal Antibody, Clone RF5R
Ab1007-100 100ug
EUR 270

RFP-Tag Mouse Monoclonal Antibody, Clone RF5R
Ab1007-101 1mg
EUR 1780

Red Fluorescent Protein (dsRed)
MBS634623-01mg 0.1mg
EUR 645

Red Fluorescent Protein (dsRed)
MBS634623-5x01mg 5x0.1mg
EUR 2750

Native Red Fluorescent Protein
R-PE-004R 1mg
EUR 398.4
Description: Store R-PE in dark at ~ 2-8 centigrade. Do not freeze

Anti-GFP (Green Fluorescent Protein) (Mouse) Texas Red Conjugated Antibody
GWB-D2D74D 1 mg Ask for price

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-005mg 0.05mg
EUR 320

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-01mg 0.1mg
EUR 410

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-5x01mg 5x0.1mg
EUR 1520

Recombinant purified Red Fluorescent Proteins (RFP/dsRed) protein control for Western blot
RFP12-C 100 ul
EUR 343.2

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N-6*HN Tag
MBS156863-005mg 0.05mg
EUR 320

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N-6*HN Tag
MBS156863-01mg 0.1mg
EUR 410

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N-6*HN Tag
MBS156863-5x01mg 5x0.1mg
EUR 1520

Anti-GFP (Green Fluorescent Protein) (Goat) Texas Red Conjugated
GWB-2921F1 1 mg Ask for price

Anti- Green Fluorescent Protein (GFP) Antibody
GWB-6A60F0 0.2 mg Ask for price

Anti- Green Fluorescent Protein (GFP) Antibody
GWB-772B93 1 mg Ask for price

Recombinant (E. coli) Red Fluorescent Proteins (RFP/dsRed) protein for ELISA or Standards (>98%)
RFP15-R 25 ug
EUR 300

Anti-GFP(Green fluorescent protein) Antibody
Y050794 100 µg
EUR 450

Recombinant Red Fluorescent Protein, His-tagged
RFP-005E 100µg
EUR 686.4
Description: At -20 centigrade.
At -80 centigrade for long-term storage.

TruStrip WBV Red Fluorescent protein (RFP) quantitation & Western Blot Validation Strips (10/pk)
WBRFP10-10 1 Pk
EUR 270

Anti- Green Fluorescent Protein (GFP) -HRP Antibody
GWB-BE899D 1 mg Ask for price

Fluorescent Protein Antibody Set
K817-6-100 each
EUR 1488

Blue Fluorescent protein (His tag)
80R-2547 100 ug
EUR 447
Description: Purified recombinant Human Blue Fluorescent protein

Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-1 each
EUR 164.4

Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-5 each
EUR 326.4

Green Fluorescent Protein Antibody
GWB-A6ABAF 0.25 mg Ask for price

Green Fluorescent Protein Antibody
GWB-B77B66 0.05 mg Ask for price

Green Fluorescent Protein Antibody
GWB-B8A68D 0.1 mg Ask for price

Green Fluorescent Protein Antibody
GWB-FF1300 0.1 mg Ask for price

Green Fluorescent protein Antibody
GWB-6A6C1C 0.1 mg Ask for price

Green Fluorescent Protein Antibody
GWB-2DAA70 0.1 ml Ask for price

Green Fluorescent Protein Antibody
GWB-21E341 1 ml Ask for price

Cherry Fluorescent Protein (His tag)
80R-2546 100 ug
EUR 475
Description: Purified recombinant Human Cherry Fluorescent Protein

Anti- Green Fluorescent Protein (GFP) -BIOTIN Antibody
GWB-A81A31 1 mg Ask for price

Goat Anti Green Fluorescent Protein Polyclonal Antibody
DPBT-68157GG 0.1 mg
EUR 518.7
Description: Goat

Blue Fluorescent Protein (BFP) Antibody
abx412394-100tests 100 tests
EUR 375

Blue Fluorescent Protein (BFP) Antibody
abx412394-50ug 50 ug
EUR 594

Cyan Fluorescent Protein (CFP) Antibody
abx412395-50ug 50 ug
EUR 594

Sheep Anti Green Fluorescent Protein Polyclonal Antibody
DPBT-68156SG 1 ml
EUR 579.6
Description: Sheep

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1065

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgEColi 0.05mg(E-Coli)
EUR 730

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgYeast 0.05mg(Yeast)
EUR 895

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-02mgEColi 0.2mg(E-Coli)
EUR 975

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-05mgEColi 0.5mg(E-Coli)
EUR 1070

Green Fluorescent Protein (GFP) Antibody
abx037713-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody
abx037713-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody
abx037714-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody
abx037714-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody
abx034623-100g 100 µg
EUR 281.25

Green Fluorescent Protein (GFP) Antibody
abx034623-400ul 400 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody
abx034623-80l 80 µl
EUR 343.2

Green Fluorescent Protein (GFP) Antibody
abx034624-100g 100 µg
EUR 525

Green Fluorescent Protein (GFP) Antibody
abx034624-100ul 100 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody
abx019093-100l 100 µl
EUR 243.75

Green Fluorescent Protein (GFP) Antibody
abx019093-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody
abx019094-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody
abx019094-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody
abx019094-80l 80 µl
EUR 243.75

Green Fluorescent Protein (GFP) Antibody
abx019099-100ug 100 ug
EUR 410.4

Green Fluorescent Protein (GFP) Antibody
abx019099-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody
abx019099-80l 80 µl
EUR 300

Green Fluorescent Protein (GFP) Antibody
20-abx020016
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Green Fluorescent Protein (GFP) Antibody
abx020016-400l 400 µl
EUR 387.5

Green Fluorescent Protein (GFP) Antibody
abx020016-80l 80 µl
EUR 300

Green Fluorescent Protein (GFP) Antibody
abx011187-100g 100 µg Ask for price

Green Fluorescent Protein (GFP) Antibody
abx011187-10g 10 µg
EUR 350

We apply time-resolved electronic and stimulated Raman spectroscopy to reveal the two species are hidden from an engineering ancestral GFP-like LEA proteins, involving semi-stuck protonated (GA ‘) and stuck deprotonated (GB’) chromophore trip to photoconversion pH = 7, 9 buffer.