fluorescent proteins can produce reactive oxygen species (ROS) on the absorption of photons through type I and II photosensitization mechanisms. KillerRed red fluorescent protein and Supernova are phototoxic protein engineered to produce ROS and used in a variety of biological applications. However, their relative quantum results and the level of ROS production is unclear, which has limited the interpretation of their effects when used in a biological system.

We cloned and purified KillerRed, Supernova, and mCherry – red fluorescent protein linked not usually considered photosensitizer – and measuring superoxide (O2 • -) and singlet oxygen (1O2) quantum results with irradiation at 561 nm. The formation of O2 • 2-hydroxyethidium –specific product (2-OHE +) was quantified by HPLC with fluorescence detection separation. In connection with the reference photosensitizer, Rose Bengal, O2 • – quantum results (ΦO2 • -) of Supernova was determined to be 1.5 × 10-3, KillerRed is 0.97 × 10-3 and 1.2 × 10-3 mCherry , At 916.5 excitation fluence J / cm2 and suitable absorption at 561 nm, Supernova, KillerRed and mCherry made 3.81, 2.38 and 1.65 pM O2 • – / min, respectively.

Using Singlet Oxygen Sensor probe Green (SOSG), we confirmed the results of quantum 1O2 (Φ1O2) for Supernova be 22.0 × 10-3, KillerRed 7.6 × 10-3 and 5.7 × 10-3 mCherry. This photosensitization characteristics of Supernova, KillerRed and mCherry improve our understanding of the relevant fluorescent protein and to improve their use as a tool for advancing our knowledge of redox biology.

Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.
Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.

Green Fluorescent protein-and Discosoma sp. Red Fluorescent Protein-Tagged Lines for Protein Marker organelle subcellular localization in Rice.

Subcellular localization of proteins is a fundamental aspect of protein function. Determine the subcellular localization is important to understand the biological function of the protein. Here, we developed a set of rice organelle marker lines, which express the organelle fluorescent marker can be used as a standard of comparison in determining the subcellular localization of the protein of interest. We build green fluorescent protein (GFP) – and / or Discosoma sp. red fluorescent protein (DsRed) -tagged organelle markers targeted to the endoplasmic reticulum (ER), mitochondria, Golgi apparatus, peroxisome, actin cytoskeleton, plastids, tonoplast, plasma membrane and nucleus, respectively.

The utility of the marker lines of rice for subcellular protein localization studies indicated by detecting OsWRKY45 local nucleus and mitochondria-related NbHxk1 in protoplasts of GFP-OsH2B and ScCOX4 line-DsRed, respectively. Using the sheath-inoculation method, followed by the live-cell imaging, we detect the co-localization of Magnaporthe oryzae PWL2: mCherry: NLS fusion with GFP marker-OsH2B core in rice epidermal cells, confirming the translocation of M. oryzae effectors into host cells PWL2 , and further demonstrates the feasibility of using line organelle markers for studying the dynamics of proteins in the cell of rice in the rice and the interactions between pathogens.

Set line organelle markers developed in this study provides a valuable resource for the study of subcellular localization of proteins in the protein fluorescent bioimaging rice.Photoconvertible have significant power and biomedical advances. However, the molecular mechanisms for light-induced processes have remained elusive because of the challenge of tracking chromophore structural dynamics during photoconversion.

Green Fluorescent Protein (GFP) Monoclonal Antibody

CAU30520-200ul 200ul
EUR 384.7

Green Fluorescent Protein (GFP) Monoclonal Antibody

CAU30797-100ul 100ul
EUR 308.2

Green Fluorescent Protein (GFP) Monoclonal Antibody

CAU30797-200ul 200ul
EUR 384.7

Monoclonal Antibody to Green Fluorescent Protein (GFP)

MAD025Ge21 100ul
EUR 321

Monoclonal Antibody to Green Fluorescent Protein (GFP)

MAD025Ge22 100ul
EUR 321

Monoclonal Antibody to Green Fluorescent Protein (GFP)

MAD025Ge51 100ul
EUR 332

Anti-RFP (Red Fluorescent Protein) Antibody

GWB-3BF397 100 ug Ask for price

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species)

4-MAD025Ge21
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP)

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), APC

4-MAD025Ge21-APC
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with APC.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), Cy3

4-MAD025Ge21-Cy3
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with Cy3.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), HRP

4-MAD025Ge21-HRP
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with HRP.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), PE

4-MAD025Ge21-PE
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with PE.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), FITC

4-MAD025Ge21-FITC
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with FITC.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), APC-Cy7

4-MAD025Ge21-APC-Cy7
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with APC-Cy7.

Green Fluorescent Protein (GFP) Monoclonal Antibody (Pan-species), Biotinylated

4-MAD025Ge21-Biotin
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Description: A Mouse monoclonal antibody against Pan-species Green Fluorescent Protein (GFP). This antibody is labeled with Biotin.

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) IgG

GFP12-M 100 ug
EUR 578.4

Red Fluorescent Protein (dsRed) Antibody

3994-100 each
EUR 451.2

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-HRP conjugate

GFP12-HRP 0.5 ml
EUR 489.6

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-FITC conjugate

GFP12-FITC 0.5 ml
EUR 489.6

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-Biotin conjugate

GFP12-BTN 0.5 ml
EUR 489.6

Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-Alk Phos (AP) conjugate

GFP12-AP 0.5 ml
EUR 489.6

RFP-Tag Mouse Monoclonal Antibody, Clone RF5R

Ab1007-100 100ug
EUR 457.2

RFP-Tag Mouse Monoclonal Antibody, Clone RF5R

Ab1007-101 1mg
EUR 2733.6

Anti-GFP (Green Fluorescent Protein) (Mouse) Texas Red Conjugated Antibody

GWB-D2D74D 1 mg Ask for price

Anti- Green Fluorescent Protein (GFP) Antibody

GWB-6A60F0 0.2 mg Ask for price

Anti- Green Fluorescent Protein (GFP) Antibody

GWB-772B93 1 mg Ask for price

Anti-GFP (Green Fluorescent Protein) (Goat) Texas Red Conjugated

GWB-2921F1 1 mg Ask for price

Anti-GFP(Green fluorescent protein) Antibody

Y050794 100 µg
EUR 450

Recombinant purified Red Fluorescent Proteins (RFP/dsRed) protein control for Western blot

RFP12-C 100 ul
EUR 343.2

Fluorescent Protein Antibody Set

K817-6-100 each
EUR 1488

Blue Fluorescent protein (His tag)

80R-2547 100 ug
EUR 447
Description: Purified recombinant Human Blue Fluorescent protein

Anti- Green Fluorescent Protein (GFP) -HRP Antibody

GWB-BE899D 1 mg Ask for price

Recombinant (E. coli) Red Fluorescent Proteins (RFP/dsRed) protein for ELISA or Standards (>98%)

RFP15-R 25 ug
EUR 300

Cherry Fluorescent Protein (His tag)

80R-2546 100 ug
EUR 475
Description: Purified recombinant Human Cherry Fluorescent Protein

Green Fluorescent Protein Antibody

GWB-21E341 1 ml Ask for price

Green Fluorescent Protein Antibody

GWB-2DAA70 0.1 ml Ask for price

Green Fluorescent Protein Antibody

GWB-A6ABAF 0.25 mg Ask for price

Green Fluorescent Protein Antibody

GWB-B77B66 0.05 mg Ask for price

Green Fluorescent Protein Antibody

GWB-B8A68D 0.1 mg Ask for price

Green Fluorescent protein Antibody

GWB-6A6C1C 0.1 mg Ask for price

Green Fluorescent Protein Antibody

GWB-FF1300 0.1 mg Ask for price

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-1 each
EUR 164.4

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-5 each
EUR 326.4

Anti- Green Fluorescent Protein (GFP) -BIOTIN Antibody

GWB-A81A31 1 mg Ask for price

TruStrip WBV Red Fluorescent protein (RFP) quantitation & Western Blot Validation Strips (10/pk)

WBRFP10-10 1 Pk
EUR 270

Blue Fluorescent Protein (BFP) Antibody

abx412394-50ug 50 ug
EUR 594

Cyan Fluorescent Protein (CFP) Antibody

abx412395-50ug 50 ug
EUR 594

Blue Fluorescent Protein (BFP) Antibody

abx412394-100tests 100 tests
EUR 375

Green Fluorescent Protein (GFP) Antibody

abx034623-400ul 400 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody

abx034623-80l 80 µl
EUR 343.2

Green Fluorescent Protein (GFP) Antibody

abx034624-100ul 100 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody

abx037713-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody

abx037714-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody

20-abx132159
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Green Fluorescent Protein (GFP) Antibody

20-abx210090
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  • 100 ug
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Green Fluorescent Protein (GFP) Antibody

20-abx210351
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  • 100 ul
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Green Fluorescent Protein (GFP) Antibody

abx233433-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

abx233434-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

abx233435-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

20-abx159519
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  • 100 ug
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Green Fluorescent Protein (GFP) Antibody

20-abx137422
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  • 100 ug
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Green Fluorescent Protein (GFP) Antibody

abx140045-01mg 0.1 mg
EUR 460.8

Green Fluorescent Protein (GFP) Antibody

20-abx118007
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Green Fluorescent Protein (GFP) Antibody

20-abx134154
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Green Fluorescent Protein (GFP) Antibody

20-abx134155
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Green Fluorescent Protein (GFP) Antibody

20-abx134156
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Green Fluorescent Protein (GFP) Antibody

20-abx134157
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Green Fluorescent Protein (GFP) Antibody

20-abx005584
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Green Fluorescent Protein (GFP) Antibody

20-abx005585
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Green Fluorescent Protein (GFP) Antibody

abx015749-100ul 100 ul
EUR 493.2

Green Fluorescent Protein (GFP) Antibody

abx019093-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody

abx019094-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody

abx019099-100ug 100 ug
EUR 410.4

Green Fluorescent Protein (GFP) Antibody

20-abx020016
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Green Fluorescent Protein (GFP), Antibody

GWB-35A90C 0.1 mg Ask for price

Green Fluorescent Protein (GFP), Antibody

GWB-6E6630 1 mg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx005584-100g 100 µg
EUR 200

Green Fluorescent Protein (GFP) Antibody

abx005585-100l 100 µl
EUR 200

Green Fluorescent Protein (GFP) Antibody

abx011187-100g 100 µg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx011187-10g 10 µg
EUR 350

Green Fluorescent Protein (GFP) Antibody

abx011187-200g 200 µg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx015749-1mg 1 mg
EUR 362.5

Green Fluorescent Protein (GFP) Antibody

abx037713-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody

abx037714-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody

abx118007-100l 100 µl Ask for price

Green Fluorescent Protein (GFP) Antibody

abx118007-20l 20 µl
EUR 287.5

Green Fluorescent Protein (GFP) Antibody

abx118007-50l 50 µl
EUR 362.5

Green Fluorescent Protein (GFP) Antibody

abx132159-100tests 100 tests
EUR 275

Green Fluorescent Protein (GFP) Antibody

abx137422-100tests 100 tests
EUR 225

Green Fluorescent Protein (GFP) Antibody

abx140045-100g 100 µg
EUR 300

Green Fluorescent Protein (GFP) Antibody

abx034623-100g 100 µg
EUR 281.25

Green Fluorescent Protein (GFP) Antibody

abx034624-100g 100 µg
EUR 525

Green Fluorescent Protein (GFP) Antibody

abx019093-100l 100 µl
EUR 243.75

Green Fluorescent Protein (GFP) Antibody

abx019094-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody

abx019094-80l 80 µl
EUR 243.75

Green Fluorescent Protein (GFP) Antibody

abx019099-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody

abx019099-80l 80 µl
EUR 300

Green Fluorescent Protein (GFP) Antibody

abx020016-400l 400 µl
EUR 387.5

Green Fluorescent Protein (GFP) Antibody

abx020016-80l 80 µl
EUR 300

Green Fluorescent Protein (GFP) Antibody

abx233433-100g 100 µg
EUR 350

Green Fluorescent Protein (GFP) Antibody

abx233434-100g 100 µg
EUR 350

Green Fluorescent Protein (GFP) Antibody

abx159519-100g 100 µg Ask for price

Green Fluorescent Protein (GFP) Antibody

abx159519-10g 10 µg
EUR 187.5

Green Fluorescent Protein (GFP) Antibody

abx159519-50g 50 µg
EUR 250

Green Fluorescent Protein (GFP) Antibody

abx210090-100l 100 µl
EUR 275

Green Fluorescent Protein (GFP) Antibody

abx210090-50l 50 µl
EUR 212.5

Green Fluorescent Protein (GFP) Antibody

abx210351-100l 100 µl
EUR 350

Green Fluorescent Protein (GFP) Antibody

abx210351-50l 50 µl
EUR 250

Green Fluorescent Protein (GFP) Antibody

abx179079-96tests 96 tests
EUR 325

Green Fluorescent Protein (GFP) Antibody

abx375279-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody

abx375281-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody

abx341010-100l 100 µl
EUR 275

Green Fluorescent Protein (GFP) Antibody

abx341010-50l 50 µl
EUR 212.5

Goat Anti Green Fluorescent Protein Polyclonal Antibody

DPBT-68157GG 0.1 mg
EUR 670.8

Sheep Anti Green Fluorescent Protein Polyclonal Antibody

DPBT-68156SG 1 ml
EUR 795.6

Anti-GFP (Green Fluorescent Protein) (Goat) Fluorescein Conjugated Antibody

GWB-FE4D27 1 mg Ask for price

Rabbit Anti Green Fluorescent Protein Polyclonal Antibody

DPBT-68159RG 50 µg
EUR 1132.8

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

EKU12468-48T 48T
EUR 644

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

EKU12468-5x96T 5x96T
EUR 4370

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

EKU12468-96T 96T
EUR 920

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

EKN53580-48T 48T
EUR 436.8

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

EKN53580-5x96T 5x96T
EUR 2964

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

EKN53580-96T 96T
EUR 624

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

abx585786-100g 100 µg
EUR 6700

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

abx585786-10g 10 µg
EUR 743.75

Anti-Green Fluorescent Protein Antibody (Anti-GFP) ELISA Kit

abx585786-50g 50 µg
EUR 3537.5

Chicken Anti Green Fluorescent Protein Polyclonal Antibody

DPBT-68158CG 0.25 mg
EUR 982.8

Anti-GFP (Green Fluorescent Protein) (Mouse) Fluorescein Conjugated Antibody

GWB-CB9D8D 1 mg Ask for price

Green Fluorescent Protein (GFP) -HRP, Antibody

GWB-E12DB1 1 mg Ask for price

Rapid antibody fluorescent labeling kit (Texas Red)

EGQ00411 1 labeing(100ug/T)
EUR 225

Green Fluorescent Protein (GFP) Antibody Pair

20-abx370572
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  • 10 × 96 tests
  • 5 × 96 tests

Green Fluorescent Protein (GFP) Antibody (FITC)

abx019260-100ug 100 ug
EUR 594

Green Fluorescent Protein (GFP) Antibody (FITC)

abx019260-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody (FITC)

abx019260-80l 80 µl
EUR 425

Green Fluorescent Protein (GFP) Antibody Pair

abx370572-100g 100 µg
EUR 2725

Green Fluorescent Protein (GFP) Antibody Pair

abx370572-50g 50 µg
EUR 1687.5

ELISA Kit for Anti-Green Fluorescent Protein Antibody (Anti-GFP)

AED025Ge 96Т
EUR 800

Enhanced Blue Fluorescent Protein (EYFP) Antibody

abx375284-96tests 96 tests
EUR 337.5

Enhanced Blue Fluorescent Protein (EBFP) Antibody

abx375286-96tests 96 tests
EUR 337.5

Enhanced Blue Fluorescent Protein (EYFP) Antibody

abx375289-96tests 96 tests
EUR 337.5

Enhanced Blue Fluorescent Protein (EBFP) Antibody

abx332838-100g 100 µg Ask for price

Enhanced Blue Fluorescent Protein (EBFP) Antibody

abx332838-20g 20 µg
EUR 362.5

Enhanced Blue Fluorescent Protein (EBFP) Antibody

abx332838-50g 50 µg Ask for price

Enhanced Green Fluorescent Protein (EGFP) Antibody

abx122699-100ug 100 ug
EUR 469.2

Enhanced Green Fluorescent Protein (EGFP) Antibody

abx137584-01mg 0.1 mg
EUR 225

Enhanced Green Fluorescent Protein (EGFP) Antibody

abx122699-100l 100 µl Ask for price

Enhanced Green Fluorescent Protein (EGFP) Antibody

abx122699-20l 20 µl
EUR 337.5

Enhanced Green Fluorescent Protein (EGFP) Antibody

abx122699-50l 50 µl Ask for price

Green Fluorescent Protein (GFP) Polyclonal Antibody

CAU29134-100ul 100ul
EUR 241

Green Fluorescent Protein (GFP) Polyclonal Antibody

CAU29134-200ul 200ul
EUR 300.7

Anti-GFP (Green Fluorescent Protein) (Mouse) Biotin Conjugated Antibody

GWB-FFB8C6 1 mg Ask for price

OASA00736-100UL - GREEN FLUORESCENT PROTEIN Antibody

OASA00736-100UL 0.1ml
EUR 659

Polyclonal Antibody to Green Fluorescent Protein (GFP)

PAD025Ge02 100ul
EUR 259

We apply time-resolved electronic and stimulated Raman spectroscopy to reveal the two species are hidden from an engineering ancestral GFP-like LEA proteins, involving semi-stuck protonated (GA ‘) and stuck deprotonated (GB’) chromophore trip to photoconversion pH = 7, 9 buffer.