Fungus Trichoderma reesei filaments are a major source of cellulolytic enzymes in the production of biofuels. Despite the economic relevance, our understanding of the secretory pathway is fragmentary. The main challenge is to visualize the dynamic behavior of secretory vesicles in living cells. To this end, we establish the location of succinate dehydrogenase locus pair as “soft-landing” site for the controlled expression of four protein-encoding genes for the fluorescent red and green neon 5 (GFPs, RFP).
Quantitative and comparative analysis of their fluorescent signals in living cells showed that the codon-optimized GFP superfolder monomer (TrmsGFP) and codon-optimized mCherry (TrmCherry) combines the highest signal intensity to significantly improve the signal-to-noise. Finally, we show that the integration of the plasmid near the locus sdi1 not affect the secretion of cellulase activity in the RUT-C30. Molecular and cell imaging tool life generated in this study will help us understand the secretory pathway in T. reesei mushroom industry.
Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.
Small nanoparticles ultra-red fluorescent protein as an exogenous probes for noninvasive in vivo imaging of tumor.
Nanoparticles are excellent imaging agents for cancer, but the variability in the chemical structure, a racemic mixture, and the addition of heavy metals preclude FDA approval in the United States. We developed an ultra-small fluorescent protein in red, named smURFP, have optical properties similar to a small-molecule Cy5, subclass heptamethine cyanine dye (Ex / Em = 642/670 nm). smURFP have a fluorescence quantum results of 18% and express well in E. coli, which is the number of grams of fluorescent proteins purified from culture in the laboratory. In this study, combined with the smURFP fluorescent protein bovine serum albumin nanoparticles fluorescent protein. The fluorescent nanoparticles with quantum results of 17% and 12-14 nm in diameter.
Far-red fluorescent protein nanoparticles noninvasively image tumors in mice living through enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticles for in vivo fluorescent imaging. nanoparticle core of these proteins should prove useful as a scaffold biomacromolecular, which can bear extended chemical modifications to the study, such as imaging in vivo of nanoparticles fluorescent protein targeted to the primary cancer and metastases, treatment theranostic, and / or dual-modality imaging with positron emission tomography for entire human imaging.
Booster, Red-Shifted Genetic Encoding Forster Resonance Energy Transfer (FRET) Biosensor Compatible Cyan Fluorescent Protein / Yellow Fluorescent Protein FRET-based biosensor and Blue Light-Responsive Optogenetic Tools.
Genetically encoded Forster resonance energy transfer (FRET) based biosensor has been developed for the visualization of molecular activity signal. At present, most of them consisting of cyan and yellow fluorescent proteins (CFP and YFP), preclude the use of some of the FRET biosensor in one cell. In addition, the FRET biosensor based CFP and YFP is not compatible with optogenetic tools that operate on light blue. To resolve this problem, here, we have developed a FRET biosensor with red-shifted excitation and emission wavelengths.
Recombinant (E. coli) Red Fluorescent Proteins (RFP/dsRed) protein for ELISA or Standards (>98%)
RFP15-R
Alpha Diagnostics
25 ug
EUR 300
Red Fluorescent Protein (RFP) Antibody
abx240273-100l
Abbexa
100 µl
Ask for price
Red Fluorescent Protein (RFP) Antibody
abx240273-50l
Abbexa
50 µl
EUR 350
Red Fluorescent Protein (RFP) Antibody
abx412396-100g
Abbexa
100 µg
EUR 450
Red Fluorescent Protein (RFP) Antibody
abx412396-50ug
Abbexa
50 ug
EUR 678
Anti-RFP (Red Fluorescent Protein) Antibody
GWB-3BF397
GenWay Biotech
100 ug
Ask for price
Red Fluorescent Protein (RFP)
MBS6508207-01mg
MyBiosource
0.1mg
EUR 770
Red Fluorescent Protein (RFP)
MBS6508207-5x01mg
MyBiosource
5x0.1mg
EUR 3320
Native Red Fluorescent Protein
R-PE-004R
Creative BioMart
1mg
EUR 398.4
Description: Store R-PE in dark at ~ 2-8 centigrade. Do not freeze
Rabbit anti Red Fluorescent Protein
MBS225348-005mg
MyBiosource
0.05mg
EUR 460
Rabbit anti Red Fluorescent Protein
MBS225348-5x005mg
MyBiosource
5x0.05mg
EUR 1905
Red Fluorescent Protein (RFP) (AP)
MBS6497151-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (AP)
MBS6497151-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (PE)
MBS6497161-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (PE)
MBS6497161-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (APC)
MBS6497152-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (APC)
MBS6497152-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (HRP)
MBS6497155-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (HRP)
MBS6497155-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (FITC)
MBS6497154-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (FITC)
MBS6497154-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (Biotin)
MBS6497153-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (Biotin)
MBS6497153-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
ABP-MAB-RT008
Allele Biotech
100 ug
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Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-0025mg
MyBiosource
0.025mg
EUR 220
Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-005mg
MyBiosource
0.05mg
EUR 260
Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-01mg
MyBiosource
0.1mg
EUR 325
Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-02mg
MyBiosource
0.2mg
EUR 450
Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-05mg
MyBiosource
0.5mg
EUR 705
RFP | Red Fluorescent Protein (clone RF5R)
AS15-3028
Agrisera AB
50 µg
EUR 259
Red Fluorescent Protein (RFP) (MaxLight 405)
MBS6497156-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (MaxLight 405)
MBS6497156-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (MaxLight 490)
MBS6497157-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (MaxLight 490)
MBS6497157-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (MaxLight 550)
MBS6497158-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (MaxLight 550)
MBS6497158-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (MaxLight 650)
MBS6497159-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (MaxLight 650)
MBS6497159-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein (RFP) (MaxLight 750)
MBS6497160-01mL
MyBiosource
0.1mL
EUR 1060
Red Fluorescent Protein (RFP) (MaxLight 750)
MBS6497160-5x01mL
MyBiosource
5x0.1mL
EUR 4620
Red Fluorescent Protein, Pre-adsorbed (RFP)
MBS6506872-01mg
MyBiosource
0.1mg
EUR 715
Red Fluorescent Protein, Pre-adsorbed (RFP)
MBS6506872-5x01mg
MyBiosource
5x0.1mg
EUR 3075
RFP | Red Fluorescent Protein (mouse monoclonal)
AS15-3033
Agrisera AB
100 µg
EUR 331
Recombinant Red Fluorescent Protein, His-tagged
RFP-005E
Creative BioMart
100µg
EUR 686.4
Description: At -20 centigrade. At -80 centigrade for long-term storage.
Anti-GFP (Green Fluorescent Protein) (Mouse) Texas Red Conjugated Antibody
GWB-D2D74D
GenWay Biotech
1 mg
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Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-1
Biovision
each
EUR 164.4
Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-5
Biovision
each
EUR 326.4
Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgBaculovirus
MyBiosource
0.05mg(Baculovirus)
EUR 1065
Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgEColi
MyBiosource
0.05mg(E-Coli)
EUR 730
Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgYeast
MyBiosource
0.05mg(Yeast)
EUR 895
Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-02mgEColi
MyBiosource
0.2mg(E-Coli)
EUR 975
Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-05mgEColi
MyBiosource
0.5mg(E-Coli)
EUR 1070
Fluorescent Protein Antibody Set
K817-6-100
Biovision
each
EUR 1488
Green Fluorescent Protein Antibody
GWB-A6ABAF
GenWay Biotech
0.25 mg
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Green Fluorescent Protein Antibody
GWB-21E341
GenWay Biotech
1 ml
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Green Fluorescent protein Antibody
GWB-6A6C1C
GenWay Biotech
0.1 mg
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Green Fluorescent Protein Antibody
GWB-2DAA70
GenWay Biotech
0.1 ml
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Green Fluorescent Protein Antibody
GWB-B77B66
GenWay Biotech
0.05 mg
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Green Fluorescent Protein Antibody
GWB-B8A68D
GenWay Biotech
0.1 mg
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Green Fluorescent Protein Antibody
GWB-FF1300
GenWay Biotech
0.1 mg
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Green Fluorescent Protein, X-adsorbed (GFP) (Texas Red)
MBS610666-1mg
MyBiosource
1mg
EUR 1145
Green Fluorescent Protein, X-adsorbed (GFP) (Texas Red)
MBS610666-5x1mg
MyBiosource
5x1mg
EUR 4990
Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-002mgBaculovirus
MyBiosource
0.02mg(Baculovirus)
EUR 1125
Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-002mgEColi
MyBiosource
0.02mg(E-Coli)
EUR 740
Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-002mgYeast
MyBiosource
0.02mg(Yeast)
EUR 905
Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-01mgEColi
MyBiosource
0.1mg(E-Coli)
EUR 885
Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-01mgYeast
MyBiosource
0.1mg(Yeast)
EUR 1055
Blue Fluorescent Protein (BFP) Antibody
abx412394-100tests
Abbexa
100 tests
EUR 375
Blue Fluorescent Protein (BFP) Antibody
abx412394-50ug
Abbexa
50 ug
EUR 594
Cyan Fluorescent Protein (CFP) Antibody
abx412395-50ug
Abbexa
50 ug
EUR 594
Anti-GFP (Green Fluorescent Protein) (Goat) Texas Red Conjugated
GWB-2921F1
GenWay Biotech
1 mg
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Rapid antibody fluorescent labeling kit (Texas Red)
EGQ00411
EnoGene
1 labeing(100ug/T)
EUR 225
Rapid antibody fluorescent labeling kit (Texas Red)
MBS8584934-01mg
MyBiosource
0.1mg
EUR 305
Rapid antibody fluorescent labeling kit (Texas Red)
MBS8584934-5x01mg
MyBiosource
5x0.1mg
EUR 1480
Green Fluorescent Protein (GFP) Antibody
abx011187-100g
Abbexa
100 µg
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Green Fluorescent Protein (GFP) Antibody
abx011187-10g
Abbexa
10 µg
EUR 350
Green Fluorescent Protein (GFP) Antibody
abx011187-200g
Abbexa
200 µg
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Green Fluorescent Protein (GFP) Antibody
abx005584-100g
Abbexa
100 µg
EUR 200
Green Fluorescent Protein (GFP) Antibody
20-abx005584
Abbexa
Ask for price
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Green Fluorescent Protein (GFP) Antibody
abx005585-100l
Abbexa
100 µl
EUR 200
Green Fluorescent Protein (GFP) Antibody
20-abx005585
Abbexa
Ask for price
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Green Fluorescent Protein (GFP) Antibody
abx015749-100ul
Abbexa
100 ul
EUR 493.2
Green Fluorescent Protein (GFP) Antibody
abx015749-1mg
Abbexa
1 mg
EUR 362.5
Green Fluorescent Protein (GFP) Antibody
abx019093-100l
Abbexa
100 µl
EUR 243.75
Green Fluorescent Protein (GFP) Antibody
abx019093-100ug
Abbexa
100 ug
EUR 385.2
Green Fluorescent Protein (GFP) Antibody
abx019094-100ug
Abbexa
100 ug
EUR 385.2
Green Fluorescent Protein (GFP) Antibody
abx019094-400l
Abbexa
400 µl
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Green Fluorescent Protein (GFP) Antibody
abx019094-80l
Abbexa
80 µl
EUR 243.75
Green Fluorescent Protein (GFP) Antibody
abx019099-100ug
Abbexa
100 ug
EUR 410.4
Green Fluorescent Protein (GFP) Antibody
abx019099-400l
Abbexa
400 µl
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Green Fluorescent Protein (GFP) Antibody
abx019099-80l
Abbexa
80 µl
EUR 300
Green Fluorescent Protein (GFP) Antibody
20-abx020016
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx020016-400l
Abbexa
400 µl
EUR 387.5
Green Fluorescent Protein (GFP) Antibody
abx020016-80l
Abbexa
80 µl
EUR 300
Green Fluorescent Protein (GFP) Antibody
abx034623-100g
Abbexa
100 µg
EUR 281.25
Green Fluorescent Protein (GFP) Antibody
abx034623-400ul
Abbexa
400 ul
EUR 627.6
Green Fluorescent Protein (GFP) Antibody
abx034623-80l
Abbexa
80 µl
EUR 343.2
Green Fluorescent Protein (GFP) Antibody
abx034624-100g
Abbexa
100 µg
EUR 525
Green Fluorescent Protein (GFP) Antibody
abx034624-100ul
Abbexa
100 ul
EUR 627.6
Green Fluorescent Protein (GFP) Antibody
abx037713-100ug
Abbexa
100 ug
EUR 469.2
Green Fluorescent Protein (GFP) Antibody
abx037713-96tests
Abbexa
96 tests
EUR 337.5
Green Fluorescent Protein (GFP) Antibody
abx037714-100ug
Abbexa
100 ug
EUR 469.2
Green Fluorescent Protein (GFP) Antibody
abx037714-96tests
Abbexa
96 tests
EUR 337.5
Green Fluorescent Protein (GFP) Antibody
abx118007-100l
Abbexa
100 µl
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Green Fluorescent Protein (GFP) Antibody
20-abx118007
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx118007-20l
Abbexa
20 µl
EUR 287.5
Green Fluorescent Protein (GFP) Antibody
abx118007-50l
Abbexa
50 µl
EUR 362.5
Green Fluorescent Protein (GFP) Antibody
abx132159-100tests
Abbexa
100 tests
EUR 275
Green Fluorescent Protein (GFP) Antibody
20-abx132159
Abbexa
Ask for price
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10 ug
50 ug
100 ug
200 ug
1 mg
Green Fluorescent Protein (GFP) Antibody
20-abx134154
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx134155
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx134156
Abbexa
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Green Fluorescent Protein (GFP) Antibody
20-abx134157
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx137422-100tests
Abbexa
100 tests
EUR 225
Green Fluorescent Protein (GFP) Antibody
20-abx137422
Abbexa
Ask for price
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Green Fluorescent Protein (GFP) Antibody
abx179079-96tests
Abbexa
96 tests
EUR 325
Green Fluorescent Protein (GFP) Antibody
abx140045-01mg
Abbexa
0.1 mg
EUR 460.8
Green Fluorescent Protein (GFP) Antibody
abx140045-100g
Abbexa
100 µg
EUR 300
Green Fluorescent Protein (GFP) Antibody
abx159519-100g
Abbexa
100 µg
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Green Fluorescent Protein (GFP) Antibody
20-abx159519
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx159519-10g
Abbexa
10 µg
EUR 187.5
Green Fluorescent Protein (GFP) Antibody
abx159519-50g
Abbexa
50 µg
EUR 250
Green Fluorescent Protein (GFP) Antibody
abx341010-100l
Abbexa
100 µl
EUR 275
Green Fluorescent Protein (GFP) Antibody
abx341010-50l
Abbexa
50 µl
EUR 212.5
Green Fluorescent Protein (GFP) Antibody
abx233433-100g
Abbexa
100 µg
EUR 350
Green Fluorescent Protein (GFP) Antibody
abx233433-100ug
Abbexa
100 ug
EUR 577.2
Green Fluorescent Protein (GFP) Antibody
abx233434-100g
Abbexa
100 µg
EUR 350
Green Fluorescent Protein (GFP) Antibody
abx233434-100ug
Abbexa
100 ug
EUR 577.2
Green Fluorescent Protein (GFP) Antibody
abx233435-100ug
Abbexa
100 ug
EUR 577.2
Green Fluorescent Protein (GFP) Antibody
abx210090-100l
Abbexa
100 µl
EUR 275
Green Fluorescent Protein (GFP) Antibody
20-abx210090
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx210090-50l
Abbexa
50 µl
EUR 212.5
Green Fluorescent Protein (GFP) Antibody
abx210351-100l
Abbexa
100 µl
EUR 350
Green Fluorescent Protein (GFP) Antibody
20-abx210351
Abbexa
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Green Fluorescent Protein (GFP) Antibody
abx210351-50l
Abbexa
50 µl
EUR 250
Green Fluorescent Protein (GFP) Antibody
abx375279-96tests
Abbexa
96 tests
EUR 337.5
Green Fluorescent Protein (GFP) Antibody
abx375281-96tests
Abbexa
96 tests
EUR 337.5
Green Fluorescent Protein (GFP), Antibody
GWB-6E6630
GenWay Biotech
1 mg
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Green Fluorescent Protein (GFP), Antibody
GWB-35A90C
GenWay Biotech
0.1 mg
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Fluorescent Protein Antibody Set, BioAssay
MBS629957-6x01mg
MyBiosource
6x0.1mg
EUR 1530
Green Fluorescent Protein (GFP) -HRP, Antibody
GWB-E12DB1
GenWay Biotech
1 mg
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Anti- Green Fluorescent Protein (GFP) Antibody
GWB-6A60F0
GenWay Biotech
0.2 mg
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Anti- Green Fluorescent Protein (GFP) Antibody
GWB-772B93
GenWay Biotech
1 mg
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Green Fluorescent Protein (GFP) Antibody (FITC)
abx019260-100ug
Abbexa
100 ug
EUR 594
Green Fluorescent Protein (GFP) Antibody (FITC)
abx019260-400l
Abbexa
400 µl
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Green Fluorescent Protein (GFP) Antibody (FITC)
abx019260-80l
Abbexa
80 µl
EUR 425
Green Fluorescent Protein (GFP) Antibody Pair
abx370572-100g
Abbexa
100 µg
EUR 2725
Green Fluorescent Protein (GFP) Antibody Pair
20-abx370572
Abbexa
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5 × 96 tests
10 × 96 tests
Green Fluorescent Protein (GFP) Antibody Pair
abx370572-50g
Abbexa
50 µg
EUR 1687.5
Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-005mg
MyBiosource
0.05mg
EUR 320
Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-01mg
MyBiosource
0.1mg
EUR 410
Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-5x01mg
MyBiosource
5x0.1mg
EUR 1520
Anti-GFP(Green fluorescent protein) Antibody
Y050794
ABM
100 µg
EUR 450
Enhanced Blue Fluorescent Protein (EBFP) Antibody
abx332838-100g
Abbexa
100 µg
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Enhanced Blue Fluorescent Protein (EBFP) Antibody
abx332838-20g
Abbexa
20 µg
EUR 362.5
Enhanced Blue Fluorescent Protein (EBFP) Antibody
abx332838-50g
Abbexa
50 µg
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We chose mKOκ and mKate2 as favorable donor and acceptor pair by calculating the distance Forster. By optimizing the fluorescent protein sequence and domain modulator of FRET biosensor, we developed a FRET biosensor backbone named “Booster”. Performance of protein kinase A (PKA) biosensor based backbone Booster (Booster-PKA) is comparable to AKAR3EV, a previously developed FRET biosensor consists CFP and YFP.