Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.
by Angelica
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Fungus Trichoderma reesei filaments are a major source of cellulolytic enzymes in the production of biofuels. Despite the economic relevance, our understanding of the secretory pathway is fragmentary. The main challenge is to visualize the dynamic behavior of secretory vesicles in living cells. To this end, we establish the location of succinate dehydrogenase locus pair as “soft-landing” site for the controlled expression of four protein-encoding genes for the fluorescent red and green neon 5 (GFPs, RFP).
Quantitative and comparative analysis of their fluorescent signals in living cells showed that the codon-optimized GFP superfolder monomer (TrmsGFP) and codon-optimized mCherry (TrmCherry) combines the highest signal intensity to significantly improve the signal-to-noise. Finally, we show that the integration of the plasmid near the locus sdi1 not affect the secretion of cellulase activity in the RUT-C30. Molecular and cell imaging tool life generated in this study will help us understand the secretory pathway in T. reesei mushroom industry.
Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.
Small nanoparticles ultra-red fluorescent protein as an exogenous probes for noninvasive in vivo imaging of tumor.
Nanoparticles are excellent imaging agents for cancer, but the variability in the chemical structure, a racemic mixture, and the addition of heavy metals preclude FDA approval in the United States. We developed an ultra-small fluorescent protein in red, named smURFP, have optical properties similar to a small-molecule Cy5, subclass heptamethine cyanine dye (Ex / Em = 642/670 nm). smURFP have a fluorescence quantum results of 18% and express well in E. coli, which is the number of grams of fluorescent proteins purified from culture in the laboratory. In this study, combined with the smURFP fluorescent protein bovine serum albumin nanoparticles fluorescent protein. The fluorescent nanoparticles with quantum results of 17% and 12-14 nm in diameter.
Far-red fluorescent protein nanoparticles noninvasively image tumors in mice living through enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticles for in vivo fluorescent imaging. nanoparticle core of these proteins should prove useful as a scaffold biomacromolecular, which can bear extended chemical modifications to the study, such as imaging in vivo of nanoparticles fluorescent protein targeted to the primary cancer and metastases, treatment theranostic, and / or dual-modality imaging with positron emission tomography for entire human imaging.
Booster, Red-Shifted Genetic Encoding Forster Resonance Energy Transfer (FRET) Biosensor Compatible Cyan Fluorescent Protein / Yellow Fluorescent Protein FRET-based biosensor and Blue Light-Responsive Optogenetic Tools.
Genetically encoded Forster resonance energy transfer (FRET) based biosensor has been developed for the visualization of molecular activity signal. At present, most of them consisting of cyan and yellow fluorescent proteins (CFP and YFP), preclude the use of some of the FRET biosensor in one cell. In addition, the FRET biosensor based CFP and YFP is not compatible with optogenetic tools that operate on light blue. To resolve this problem, here, we have developed a FRET biosensor with red-shifted excitation and emission wavelengths.
Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: rGFP Aequorea victoria produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-238 a.a.) and having a molecular mass of 26.8 kDa.;rGFP is purified by proprietary chromatographic techniques.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Absolute Mag Magnetic Particles, Fluorescent Nile Red
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of General Green Fluorescent Protein (GFP) in Biological agents.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of General Green Fluorescent Protein (GFP) in Biological agents.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of General Green Fluorescent Protein (GFP) in Biological agents.
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We chose mKOκ and mKate2 as favorable donor and acceptor pair by calculating the distance Forster. By optimizing the fluorescent protein sequence and domain modulator of FRET biosensor, we developed a FRET biosensor backbone named “Booster”. Performance of protein kinase A (PKA) biosensor based backbone Booster (Booster-PKA) is comparable to AKAR3EV, a previously developed FRET biosensor consists CFP and YFP.
