Fungus Trichoderma reesei filaments are a major source of cellulolytic enzymes in the production of biofuels. Despite the economic relevance, our understanding of the secretory pathway is fragmentary. The main challenge is to visualize the dynamic behavior of secretory vesicles in living cells. To this end, we establish the location of succinate dehydrogenase locus pair as “soft-landing” site for the controlled expression of four protein-encoding genes for the fluorescent red and green neon 5 (GFPs, RFP).

Quantitative and comparative analysis of their fluorescent signals in living cells showed that the codon-optimized GFP superfolder monomer (TrmsGFP) and codon-optimized mCherry (TrmCherry) combines the highest signal intensity to significantly improve the signal-to-noise. Finally, we show that the integration of the plasmid near the locus sdi1 not affect the secretion of cellulase activity in the RUT-C30. Molecular and cell imaging tool life generated in this study will help us understand the secretory pathway in T. reesei mushroom industry.

Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.
Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.

Small nanoparticles ultra-red fluorescent protein as an exogenous probes for noninvasive in vivo imaging of tumor.

Nanoparticles are excellent imaging agents for cancer, but the variability in the chemical structure, a racemic mixture, and the addition of heavy metals preclude FDA approval in the United States. We developed an ultra-small fluorescent protein in red, named smURFP, have optical properties similar to a small-molecule Cy5, subclass heptamethine cyanine dye (Ex / Em = 642/670 nm). smURFP have a fluorescence quantum results of 18% and express well in E. coli, which is the number of grams of fluorescent proteins purified from culture in the laboratory. In this study, combined with the smURFP fluorescent protein bovine serum albumin nanoparticles fluorescent protein. The fluorescent nanoparticles with quantum results of 17% and 12-14 nm in diameter.

Far-red fluorescent protein nanoparticles noninvasively image tumors in mice living through enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticles for in vivo fluorescent imaging. nanoparticle core of these proteins should prove useful as a scaffold biomacromolecular, which can bear extended chemical modifications to the study, such as imaging in vivo of nanoparticles fluorescent protein targeted to the primary cancer and metastases, treatment theranostic, and / or dual-modality imaging with positron emission tomography for entire human imaging.

Booster, Red-Shifted Genetic Encoding Forster Resonance Energy Transfer (FRET) Biosensor Compatible Cyan Fluorescent Protein / Yellow Fluorescent Protein FRET-based biosensor and Blue Light-Responsive Optogenetic Tools.

Genetically encoded Forster resonance energy transfer (FRET) based biosensor has been developed for the visualization of molecular activity signal. At present, most of them consisting of cyan and yellow fluorescent proteins (CFP and YFP), preclude the use of some of the FRET biosensor in one cell. In addition, the FRET biosensor based CFP and YFP is not compatible with optogenetic tools that operate on light blue. To resolve this problem, here, we have developed a FRET biosensor with red-shifted excitation and emission wavelengths.

RFP (dsRed) Antibody

3993-100
EUR 430

RFP (dsRed) Antibody

3993-30T
EUR 185

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-1
EUR 137

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-5
EUR 272

EZ-Red? E. coli Fluorescent Particles

M1202-500
EUR 343

EZ-Red? Zymosan A Fluorescent Particles

M1204-500
EUR 343

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody

ABP-MAB-RT008 100 ug Ask for price

DiagNano Red Fluorescent Silica Nanoparticles, 10 nm

DNG-L007 10 mL
EUR 845

DiagNano Red Fluorescent Silica Nanoparticles, 30 nm

DNG-L009 10 mL
EUR 725

DiagNano Red Fluorescent Silica Nanoparticles, 50 nm

DNG-L010 10 mL
EUR 679

DiagNano Red Fluorescent Silica Nanoparticles, 70 nm

DNG-L012 10 mL
EUR 611

DiagNano Red Fluorescent Silica Nanoparticles, 100 nm

DNG-L016 10 mL
EUR 611

DiagNano Red Fluorescent Silica Nanoparticles, 200 nm

DNG-L017 10 mL
EUR 611

DiagNano Red Fluorescent Silica Particles, 300 nm

DNG-L020 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 500 nm

DNG-L025 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 1 μm

DNG-L026 10 mL
EUR 632

DiagNano Red Fluorescent Silica Particles, 1.5 μm

DNG-L029 10 mL
EUR 632

DiagNano Red Fluorescent Silica Particles, 3 μm

DNG-L032 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 5 μm

DNG-L033 10 mL
EUR 627

DiagNano Red Fluorescent Silica Particles, 10 μm

DNG-L036 10 mL
EUR 637

DiagNano Red Fluorescent Silica Particles, 15 μm

DNG-L038 10 mL
EUR 637

DiagNano Red Fluorescent Silica Particles, 20 μm

DNG-L039 10 mL
EUR 637

Human Mesenchymal Stem Cells-Red Fluorescent-Labeled

SC00A3 500000
EUR 962

Absolute Mag Magnetic Particles, Fluorescent Nile Red

WHM-S134 2 mL
EUR 627

DsRed- rab7 WT

PVT10355 2 ug
EUR 266

DsRed- Rab5 WT

PVT10499 2 ug
EUR 266

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 30 nm

DNG-L042 10 mL
EUR 819

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 50 nm

DNG-L043 10 mL
EUR 793

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 70 nm

DNG-L045 10 mL
EUR 788

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 100 nm

DNG-L048 10 mL
EUR 684

DiagNano Red Fluorescent, Amine Silica Particles, 200 nm

DNG-L049 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 300 nm

DNG-L052 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 500 nm

DNG-L057 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 1 μm

DNG-L058 10 mL
EUR 684

DiagNano Red Fluorescent, Amine Silica Particles, 1.5 μm

DNG-L061 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 3 μm

DNG-L065 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 4 μm

DNG-L068 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 5 μm

DNG-L069 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 10 μm

DNG-L074 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 15 μm

DNG-L077 10 mL
EUR 679

DiagNano Red Fluorescent, Amine Silica Particles, 20 μm

DNG-L078 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 30 nm

DNG-L082 10 mL
EUR 793

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 50 nm

DNG-L083 10 mL
EUR 767

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 70 nm

DNG-L085 10 mL
EUR 762

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 100 nm

DNG-L088 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 200 nm

DNG-L089 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 300 nm

DNG-L092 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 500 nm

DNG-L097 10 mL
EUR 684

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1 μm

DNG-L098 10 mL
EUR 684

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1.5 μm

DNG-L101 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 3 μm

DNG-L105 10 mL
EUR 684

DiagNano Red Fluorescent, Carboxyl Silica Particles, 4 μm

DNG-L108 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 5 μm

DNG-L109 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 10 μm

DNG-L114 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 15 μm

DNG-L117 10 mL
EUR 679

DiagNano Red Fluorescent, Carboxyl Silica Particles, 20 μm

DNG-L118 10 mL
EUR 679

Absolute Mag Streptavidin Magnetic Particles, Fluorescent Nile Red

WHM-S119 2 mL
EUR 736

Absolute Mag Biotin Magnetic Particles, Fluorescent Nile Red

WHM-S127 5 mL
EUR 788

Absolute Mag Amine Magnetic Particles, Fluorescent Nile Red

WHM-S138 2 mL
EUR 621

pIRES2- DsRed- Express Plasmid

PVT1229 2 ug
EUR 241

Absolute Mag Carboxyl Magnetic Particles, Ferromagnetic, Fluorescent Nile Red

WHM-S011 2 mL
EUR 621

Absolute Mag Protein G Magnetic Polystyrene particles, Fluorescent Red, 2 µm

WHM-M079 1 mL
EUR 788

Fluorescent Protein Antibody Set

K817-6-100
EUR 1240

pBRPyCAG- Mettl3- dsRed- IRES- puro

PVT10449 2 ug
EUR 301

pLVX- DsRed- Monomer- N1 Plasmid

PVT2312 2 ug
EUR 301

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.04-0.06 µm

DNM-L008 2 mL
EUR 657

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.1-0.3 µm

DNM-L009 2 mL
EUR 657

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L010 2 mL
EUR 657

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 1.7-2.2 µm

DNM-L036 2 mL
EUR 663

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 2.5-3.4 µm

DNM-L037 2 mL
EUR 729

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 3.5-4.5 µm

DNM-L038 2 mL
EUR 729

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 6.0-7.9 µm

DNM-L039 2 mL
EUR 729

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 8.0-9.9 µm

DNM-L040 2 mL
EUR 840

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 10.0-14.0 µm

DNM-L041 2 mL
EUR 823

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 15.0-19.0 µm

DNM-L042 2 mL
EUR 840

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.04-0.08 µm

DNM-L070 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.09-0.3 µm

DNM-L071 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.4-0.6 µm

DNM-L072 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L073 2 mL
EUR 657

Absolute Mag Amine Magnetic Dextran Nanoparticles, Fluorescent Red, 20 nm

WHM-C156 5 mL
EUR 789

Absolute Mag Plain Magnetic Dextran Particles, Fluorescent Red, 100 nm

WHM-M100 5 mL
EUR 1027

Absolute Mag Amine Magnetic Dextran Particles, Fluorescent Red, 100 nm

WHM-M101 5 mL
EUR 1027

RED antibody

22518-100ul 100ul
EUR 390

RED Antibody

20-abx249463
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

RED Antibody

DF4352 200ul
EUR 304
Description: RED Antibody detects endogenous levels of total RED.

RED Antibody

ABD4352 100 ug
EUR 438

RED antibody

70R-13184 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal RED antibody

Fluorescent

FPAK-3058--9K 9X1 mL
EUR 563
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

DiagPoly Avidin Coated Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L096 5 mL
EUR 663

DiagPoly Biotin Coated Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L103 5 mL
EUR 663

DiagPoly Biotin Coated Fluorescent Polystyrene Particles, Nile Red, 7.0-7.9 µm

DNM-L104 5 mL
EUR 829

Absolute Mag Amine Magnetic Nanoparticles, Dextran Coated, Fluorescent Red, 100 nm

WHF-G117 5 mL
EUR 812

Absolute Mag Plain Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M097 5 mL
EUR 1027

Absolute Mag Amine Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M098 5 mL
EUR 1027

Absolute Mag Streptavidin Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M099 1 mL
EUR 835

Absolute Mag Amine Magnetic Particles, Fluorescent Nile Red, PEG Spacer Arm

WHM-S139 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.2-0.39 μm

WHM-S140 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.4-0.69 μm

WHM-S142 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.7-0.9 μm

WHM-S144 2 mL
EUR 621

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 1.0-1.4 μm

WHM-S146 2 mL
EUR 585

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 4.0-4.9 μm

WHM-S156 2 mL
EUR 658

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 8.0-9.9 μm

WHM-S162 2 mL
EUR 694

TruStrip WBV Red Fluorescent protein (RFP) quantitation & Western Blot Validation Strips (10/pk)

WBRFP10-10 1 Pk
EUR 225

Green Fluorescent Protein

MO22184 100 ul
EUR 409

Green Fluorescent Protein

CH22124 100 ul of 1 mg/ml
EUR 435

Cyan Fluorescent Protein

30R-2808 100 ug
EUR 527
Description: Purified recombinant Human Cyan Fluorescent Protein

Fluorescent Protein Set

K816-6-100
EUR 1240

mCherry Fluorescent Protein

4993-100
EUR 408

mCherry Fluorescent Protein

4993-1000
EUR 2621

mCherry Fluorescent Protein

4993-5000
EUR 7646

Cyan Fluorescent Protein

4996-100
EUR 376

Cyan Fluorescent Protein

4996-1000
EUR 2388

Cyan Fluorescent Protein

4996-5000
EUR 6520

Yellow Fluorescent Protein

4998-100
EUR 392

Yellow Fluorescent Protein

4998-1000
EUR 2513

Yellow Fluorescent Protein

4998-5000
EUR 6846

Green Fluorescent Protein (GFP) Antibody

20-abx118007
  • EUR 551.00
  • EUR 481.00
  • 100 ul
  • 50 ul

Green Fluorescent Protein (GFP) Antibody

abx034623-400ul 400 ul
EUR 523

Green Fluorescent Protein (GFP) Antibody

abx034623-80l 80 µl
EUR 286

Green Fluorescent Protein (GFP) Antibody

abx034624-100ul 100 ul
EUR 523

Green Fluorescent Protein (GFP) Antibody

abx037713-100ug 100 ug
EUR 391

Green Fluorescent Protein (GFP) Antibody

abx037714-100ug 100 ug
EUR 391

Green Fluorescent Protein (GFP) Antibody

20-abx132159
  • EUR 300.00
  • EUR 133.00
  • EUR 801.00
  • EUR 425.00
  • EUR 258.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

20-abx134154
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134155
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134156
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134157
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

abx140045-01mg 0.1 mg
EUR 384

Green Fluorescent Protein (GFP) Antibody

20-abx137422
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Green Fluorescent Protein (GFP) Antibody

20-abx005584
  • EUR 314.00
  • EUR 411.00
  • EUR 258.00
  • 100 ul
  • 200 ul
  • 50 ul

Green Fluorescent Protein (GFP) Antibody

20-abx005585
  • EUR 314.00
  • EUR 411.00
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody

abx015749-100ul 100 ul
EUR 411

Green Fluorescent Protein (GFP) Antibody

abx019093-100ug 100 ug
EUR 321

Green Fluorescent Protein (GFP) Antibody

abx019094-100ug 100 ug
EUR 321

Green Fluorescent Protein (GFP) Antibody

abx019099-100ug 100 ug
EUR 342

Green Fluorescent Protein (GFP) Antibody

20-abx020016
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Blue Fluorescent Protein (BFP) Antibody

abx412394-50ug 50 ug
EUR 495

Cyan Fluorescent Protein (CFP) Antibody

abx412395-50ug 50 ug
EUR 495

Green Fluorescent Protein (GFP) Antibody

20-abx210090
  • EUR 328.00
  • EUR 272.00
  • 100 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

20-abx159519
  • EUR 272.00
  • EUR 230.00
  • 100 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

abx233433-100ug 100 ug
EUR 481

Green Fluorescent Protein (GFP) Antibody

abx233434-100ug 100 ug
EUR 481

Green Fluorescent Protein (GFP) Antibody

abx233435-100ug 100 ug
EUR 481

Green Fluorescent Protein (GFP) Antibody

20-abx210351
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red Low Intensity, 1.7-2.2 µm

DNM-L032 2 mL
EUR 663

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red Low Intensity, 6.0-7.9 µm

DNM-L033 2 mL
EUR 729

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red High Intensity, 1.7-2.2 µm

DNM-L034 2 mL
EUR 663

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red High Intensity, 10.0-14.0 µm

DNM-L035 2 mL
EUR 823

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red Low Intensity, 1.7-2.2 µm

DNM-L074 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red Medium Intensity, 5.0-5.9 µm

DNM-L075 2 mL
EUR 657

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red High Intensity, 5.0-5.9 µm

DNM-L076 2 mL
EUR 657

Absolute Mag Streptavidin Magnetic Dextran Nanoparticles, Dextran Coated, Fluorescent Red, 300 nm

WHM-G258 1 mL
EUR 892

Absolute Mag Plain Magnetic Particles, Crosslinked Dextran-coated, Fluorescent Red, 100 nm

WHM-M092 5 mL
EUR 1027

Absolute Mag Streptavidin Magnetic Particles, Crosslinked Dextran-coated, Fluorescent Red, 100 nm

WHM-M093 1 mL
EUR 835

Absolute Mag Plain Magnetic Particles, Hydroxyethyl Starch, Fluorescent Far Red, 100 nm

WHM-M094 5 mL
EUR 1027

Absolute Mag Amine Magnetic Particles, Hydroxyethyl Starch, Fluorescent Far Red, 100 nm

WHM-M095 5 mL
EUR 1027

We chose mKOκ and mKate2 as favorable donor and acceptor pair by calculating the distance Forster. By optimizing the fluorescent protein sequence and domain modulator of FRET biosensor, we developed a FRET biosensor backbone named “Booster”. Performance of protein kinase A (PKA) biosensor based backbone Booster (Booster-PKA) is comparable to AKAR3EV, a previously developed FRET biosensor consists CFP and YFP.