Fungus Trichoderma reesei filaments are a major source of cellulolytic enzymes in the production of biofuels. Despite the economic relevance, our understanding of the secretory pathway is fragmentary. The main challenge is to visualize the dynamic behavior of secretory vesicles in living cells. To this end, we establish the location of succinate dehydrogenase locus pair as “soft-landing” site for the controlled expression of four protein-encoding genes for the fluorescent red and green neon 5 (GFPs, RFP).

Quantitative and comparative analysis of their fluorescent signals in living cells showed that the codon-optimized GFP superfolder monomer (TrmsGFP) and codon-optimized mCherry (TrmCherry) combines the highest signal intensity to significantly improve the signal-to-noise. Finally, we show that the integration of the plasmid near the locus sdi1 not affect the secretion of cellulase activity in the RUT-C30. Molecular and cell imaging tool life generated in this study will help us understand the secretory pathway in T. reesei mushroom industry.

Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.
Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.

Small nanoparticles ultra-red fluorescent protein as an exogenous probes for noninvasive in vivo imaging of tumor.

Nanoparticles are excellent imaging agents for cancer, but the variability in the chemical structure, a racemic mixture, and the addition of heavy metals preclude FDA approval in the United States. We developed an ultra-small fluorescent protein in red, named smURFP, have optical properties similar to a small-molecule Cy5, subclass heptamethine cyanine dye (Ex / Em = 642/670 nm). smURFP have a fluorescence quantum results of 18% and express well in E. coli, which is the number of grams of fluorescent proteins purified from culture in the laboratory. In this study, combined with the smURFP fluorescent protein bovine serum albumin nanoparticles fluorescent protein. The fluorescent nanoparticles with quantum results of 17% and 12-14 nm in diameter.

Far-red fluorescent protein nanoparticles noninvasively image tumors in mice living through enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticles for in vivo fluorescent imaging. nanoparticle core of these proteins should prove useful as a scaffold biomacromolecular, which can bear extended chemical modifications to the study, such as imaging in vivo of nanoparticles fluorescent protein targeted to the primary cancer and metastases, treatment theranostic, and / or dual-modality imaging with positron emission tomography for entire human imaging.

Booster, Red-Shifted Genetic Encoding Forster Resonance Energy Transfer (FRET) Biosensor Compatible Cyan Fluorescent Protein / Yellow Fluorescent Protein FRET-based biosensor and Blue Light-Responsive Optogenetic Tools.

Genetically encoded Forster resonance energy transfer (FRET) based biosensor has been developed for the visualization of molecular activity signal. At present, most of them consisting of cyan and yellow fluorescent proteins (CFP and YFP), preclude the use of some of the FRET biosensor in one cell. In addition, the FRET biosensor based CFP and YFP is not compatible with optogenetic tools that operate on light blue. To resolve this problem, here, we have developed a FRET biosensor with red-shifted excitation and emission wavelengths.

RFP (dsRed) Antibody

3993-100 each
EUR 516

RFP (dsRed) Antibody

3993-30T each
EUR 222

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-1 each
EUR 164.4

Red Fluorescent Protein - R-PE (R-Phycoerythrin)

6005-5 each
EUR 326.4

EZ-Red? E. coli Fluorescent Particles

M1202-500 each
EUR 411.6

EZ-Red? Zymosan A Fluorescent Particles

M1204-500 each
EUR 411.6

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody

ABP-MAB-RT008 100 ug Ask for price

DsRed- rab7 WT

PVT10355 2 ug
EUR 319.2

DsRed- Rab5 WT

PVT10499 2 ug
EUR 319.2

DiagNano Red Fluorescent Silica Nanoparticles, 10 nm

DNG-L007 10 mL
EUR 1014

DiagNano Red Fluorescent Silica Nanoparticles, 30 nm

DNG-L009 10 mL
EUR 870

DiagNano Red Fluorescent Silica Nanoparticles, 50 nm

DNG-L010 10 mL
EUR 814.8

DiagNano Red Fluorescent Silica Nanoparticles, 70 nm

DNG-L012 10 mL
EUR 733.2

DiagNano Red Fluorescent Silica Nanoparticles, 100 nm

DNG-L016 10 mL
EUR 733.2

DiagNano Red Fluorescent Silica Nanoparticles, 200 nm

DNG-L017 10 mL
EUR 733.2

DiagNano Red Fluorescent Silica Particles, 300 nm

DNG-L020 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 500 nm

DNG-L025 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 1 μm

DNG-L026 10 mL
EUR 758.4

DiagNano Red Fluorescent Silica Particles, 1.5 μm

DNG-L029 10 mL
EUR 758.4

DiagNano Red Fluorescent Silica Particles, 3 μm

DNG-L032 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 5 μm

DNG-L033 10 mL
EUR 752.4

DiagNano Red Fluorescent Silica Particles, 10 μm

DNG-L036 10 mL
EUR 764.4

DiagNano Red Fluorescent Silica Particles, 15 μm

DNG-L038 10 mL
EUR 764.4

DiagNano Red Fluorescent Silica Particles, 20 μm

DNG-L039 10 mL
EUR 764.4

Human Mesenchymal Stem Cells-Red Fluorescent-Labeled

SC00A3 500000
EUR 1154.4

Absolute Mag Magnetic Particles, Fluorescent Nile Red

WHM-S134 2 mL
EUR 752.4

pIRES2- DsRed- Express Plasmid

PVT1229 2 ug
EUR 289.2

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 30 nm

DNG-L042 10 mL
EUR 982.8

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 50 nm

DNG-L043 10 mL
EUR 951.6

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 70 nm

DNG-L045 10 mL
EUR 945.6

DiagNano Red Fluorescent, Amine Silica Nanoparticles, 100 nm

DNG-L048 10 mL
EUR 820.8

DiagNano Red Fluorescent, Amine Silica Particles, 200 nm

DNG-L049 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 300 nm

DNG-L052 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 500 nm

DNG-L057 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 1 μm

DNG-L058 10 mL
EUR 820.8

DiagNano Red Fluorescent, Amine Silica Particles, 1.5 μm

DNG-L061 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 3 μm

DNG-L065 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 4 μm

DNG-L068 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 5 μm

DNG-L069 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 10 μm

DNG-L074 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 15 μm

DNG-L077 10 mL
EUR 814.8

DiagNano Red Fluorescent, Amine Silica Particles, 20 μm

DNG-L078 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 30 nm

DNG-L082 10 mL
EUR 951.6

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 50 nm

DNG-L083 10 mL
EUR 920.4

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 70 nm

DNG-L085 10 mL
EUR 914.4

DiagNano Red Fluorescent, Carboxyl Silica Nanoparticles, 100 nm

DNG-L088 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 200 nm

DNG-L089 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 300 nm

DNG-L092 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 500 nm

DNG-L097 10 mL
EUR 820.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1 μm

DNG-L098 10 mL
EUR 820.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 1.5 μm

DNG-L101 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 3 μm

DNG-L105 10 mL
EUR 820.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 4 μm

DNG-L108 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 5 μm

DNG-L109 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 10 μm

DNG-L114 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 15 μm

DNG-L117 10 mL
EUR 814.8

DiagNano Red Fluorescent, Carboxyl Silica Particles, 20 μm

DNG-L118 10 mL
EUR 814.8

Absolute Mag Streptavidin Magnetic Particles, Fluorescent Nile Red

WHM-S119 2 mL
EUR 883.2

Absolute Mag Biotin Magnetic Particles, Fluorescent Nile Red

WHM-S127 5 mL
EUR 945.6

Absolute Mag Amine Magnetic Particles, Fluorescent Nile Red

WHM-S138 2 mL
EUR 745.2

pLVX- DsRed- Monomer- N1 Plasmid

PVT2312 2 ug
EUR 361.2

pBRPyCAG- Mettl3- dsRed- IRES- puro

PVT10449 2 ug
EUR 361.2

Absolute Mag Carboxyl Magnetic Particles, Ferromagnetic, Fluorescent Nile Red

WHM-S011 2 mL
EUR 745.2

Fluorescent Protein Antibody Set

K817-6-100 each
EUR 1488

Absolute Mag Protein G Magnetic Polystyrene particles, Fluorescent Red, 2 µm

WHM-M079 1 mL
EUR 945.6

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.04-0.06 µm

DNM-L008 2 mL
EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.1-0.3 µm

DNM-L009 2 mL
EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L010 2 mL
EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 1.7-2.2 µm

DNM-L036 2 mL
EUR 795.6

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 2.5-3.4 µm

DNM-L037 2 mL
EUR 874.8

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 3.5-4.5 µm

DNM-L038 2 mL
EUR 874.8

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 6.0-7.9 µm

DNM-L039 2 mL
EUR 874.8

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 8.0-9.9 µm

DNM-L040 2 mL
EUR 1008

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 10.0-14.0 µm

DNM-L041 2 mL
EUR 987.6

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 15.0-19.0 µm

DNM-L042 2 mL
EUR 1008

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.04-0.08 µm

DNM-L070 2 mL
EUR 788.4

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.09-0.3 µm

DNM-L071 2 mL
EUR 788.4

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.4-0.6 µm

DNM-L072 2 mL
EUR 788.4

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L073 2 mL
EUR 788.4

Absolute Mag Amine Magnetic Dextran Nanoparticles, Fluorescent Red, 20 nm

WHM-C156 5 mL
EUR 946.8

Absolute Mag Plain Magnetic Dextran Particles, Fluorescent Red, 100 nm

WHM-M100 5 mL
EUR 1232.4

Absolute Mag Amine Magnetic Dextran Particles, Fluorescent Red, 100 nm

WHM-M101 5 mL
EUR 1232.4

Fluorescent

FPAK-3058--9K 9X1 mL
EUR 675.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Green Fluorescent Protein

CH22124 100 ul of 1 mg/ml
EUR 522

Cyan Fluorescent Protein

30R-2808 100 ug
EUR 632.4
Description: Purified recombinant Human Cyan Fluorescent Protein

Green Fluorescent Protein

MO22184 100 ul
EUR 490.8

mCherry Fluorescent Protein

4993-100 each
EUR 489.6

mCherry Fluorescent Protein

4993-1000 each
EUR 3145.2

mCherry Fluorescent Protein

4993-5000 each
EUR 9175.2

Cyan Fluorescent Protein

4996-100 each
EUR 451.2

Cyan Fluorescent Protein

4996-1000 each
EUR 2865.6

Cyan Fluorescent Protein

4996-5000 each
EUR 7824

Yellow Fluorescent Protein

4998-100 each
EUR 470.4

Yellow Fluorescent Protein

4998-1000 each
EUR 3015.6

Yellow Fluorescent Protein

4998-5000 each
EUR 8215.2

Fluorescent Protein Set

K816-6-100 each
EUR 1488

Green Fluorescent Protein (GFP) Antibody

20-abx159519
  • EUR 326.40
  • EUR 276.00
  • 100 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

20-abx132159
  • EUR 360.00
  • EUR 159.60
  • EUR 961.20
  • EUR 510.00
  • EUR 309.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

20-abx134154
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134155
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134156
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx134157
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Green Fluorescent Protein (GFP) Antibody

20-abx137422
  • EUR 844.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 20 ug
  • 5 ug

Green Fluorescent Protein (GFP) Antibody

abx140045-01mg 0.1 mg
EUR 460.8

Green Fluorescent Protein (GFP) Antibody

20-abx118007
  • EUR 661.20
  • EUR 577.20
  • 100 ul
  • 50 ul

Green Fluorescent Protein (GFP) Antibody

20-abx005584
  • EUR 376.80
  • EUR 493.20
  • EUR 309.60
  • 100 ul
  • 200 ul
  • 50 ul

Green Fluorescent Protein (GFP) Antibody

20-abx005585
  • EUR 376.80
  • EUR 493.20
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody

abx015749-100ul 100 ul
EUR 493.2

Green Fluorescent Protein (GFP) Antibody

abx019093-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody

abx019094-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody

abx019099-100ug 100 ug
EUR 410.4

Green Fluorescent Protein (GFP) Antibody

20-abx020016
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

abx037713-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody

abx037714-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody

abx034623-400ul 400 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody

abx034623-80l 80 µl
EUR 343.2

Green Fluorescent Protein (GFP) Antibody

abx034624-100ul 100 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody

20-abx210090
  • EUR 393.60
  • EUR 326.40
  • 100 ug
  • 50 ug

Green Fluorescent Protein (GFP) Antibody

20-abx210351
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Green Fluorescent Protein (GFP) Antibody

abx233433-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

abx233434-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody

abx233435-100ug 100 ug
EUR 577.2

Blue Fluorescent Protein (BFP) Antibody

abx412394-50ug 50 ug
EUR 594

Cyan Fluorescent Protein (CFP) Antibody

abx412395-50ug 50 ug
EUR 594

TruStrip WBV Red Fluorescent protein (RFP) quantitation & Western Blot Validation Strips (10/pk)

WBRFP10-10 1 Pk
EUR 270

DiagPoly Avidin Coated Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L096 5 mL
EUR 795.6

DiagPoly Biotin Coated Fluorescent Polystyrene Particles, Nile Red, 0.7-0.9 µm

DNM-L103 5 mL
EUR 795.6

DiagPoly Biotin Coated Fluorescent Polystyrene Particles, Nile Red, 7.0-7.9 µm

DNM-L104 5 mL
EUR 994.8

Absolute Mag Amine Magnetic Nanoparticles, Dextran Coated, Fluorescent Red, 100 nm

WHF-G117 5 mL
EUR 974.4

Absolute Mag Plain Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M097 5 mL
EUR 1232.4

Absolute Mag Amine Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M098 5 mL
EUR 1232.4

Absolute Mag Streptavidin Magnetic Particles, Hydroxyethyl Starch, Fluorescent Red, 100 nm

WHM-M099 1 mL
EUR 1002

Absolute Mag Amine Magnetic Particles, Fluorescent Nile Red, PEG Spacer Arm

WHM-S139 2 mL
EUR 745.2

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.2-0.39 μm

WHM-S140 2 mL
EUR 745.2

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.4-0.69 μm

WHM-S142 2 mL
EUR 745.2

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 0.7-0.9 μm

WHM-S144 2 mL
EUR 745.2

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 1.0-1.4 μm

WHM-S146 2 mL
EUR 702

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 4.0-4.9 μm

WHM-S156 2 mL
EUR 789.6

Absolute Mag Carboxyl Magnetic Particles, Fluorescent Nile Red, 8.0-9.9 μm

WHM-S162 2 mL
EUR 832.8

RED Antibody

DF4352 200ul
EUR 420

RED antibody

70R-13184 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal RED antibody

RED antibody

22518-100ul 100ul
EUR 468

RED Antibody

ABD4352 100 ug
EUR 525.6

RED Antibody

20-abx249463
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

pMSCV- loxp- dsRed- loxp- eGFP- Puro- WPRE

PVT11052 2 ug
EUR 361.2

Green Fluorescent Protein (GFP)

CH23105 400 ul
EUR 913.2

Recombinant Green Fluorescent Protein

7-06112 10µg Ask for price

Recombinant Green Fluorescent Protein

7-06113 50µg Ask for price

Recombinant Green Fluorescent Protein

7-06114 1mg Ask for price

Green Fluorescent Protein (GFP)

MO22190 100 ul
EUR 490.8

Protein A Fluorescent Particles

PAFP-40052-5 5 mL
EUR 438
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Protein G Fluorescent Particles

PGFP-0552-5 5 mL
EUR 273.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Protein G Fluorescent Particles

PGFP-0556-5 5 mL
EUR 273.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Protein G Fluorescent Particles

PGFP-0558-5 5 mL
EUR 273.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Protein G Fluorescent Particles

PGFP-0562-5 5 mL
EUR 273.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Protein G Fluorescent Particles

PGFP-40052--5 5 mL
EUR 438
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

We chose mKOκ and mKate2 as favorable donor and acceptor pair by calculating the distance Forster. By optimizing the fluorescent protein sequence and domain modulator of FRET biosensor, we developed a FRET biosensor backbone named “Booster”. Performance of protein kinase A (PKA) biosensor based backbone Booster (Booster-PKA) is comparable to AKAR3EV, a previously developed FRET biosensor consists CFP and YFP.