Fungus Trichoderma reesei filaments are a major source of cellulolytic enzymes in the production of biofuels. Despite the economic relevance, our understanding of the secretory pathway is fragmentary. The main challenge is to visualize the dynamic behavior of secretory vesicles in living cells. To this end, we establish the location of succinate dehydrogenase locus pair as “soft-landing” site for the controlled expression of four protein-encoding genes for the fluorescent red and green neon 5 (GFPs, RFP).

Quantitative and comparative analysis of their fluorescent signals in living cells showed that the codon-optimized GFP superfolder monomer (TrmsGFP) and codon-optimized mCherry (TrmCherry) combines the highest signal intensity to significantly improve the signal-to-noise. Finally, we show that the integration of the plasmid near the locus sdi1 not affect the secretion of cellulase activity in the RUT-C30. Molecular and cell imaging tool life generated in this study will help us understand the secretory pathway in T. reesei mushroom industry.

Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.
Optimised red- and green-fluorescent proteins for live cell imaging in the industrial enzyme-producing fungus Trichoderma reesei.

Small nanoparticles ultra-red fluorescent protein as an exogenous probes for noninvasive in vivo imaging of tumor.

Nanoparticles are excellent imaging agents for cancer, but the variability in the chemical structure, a racemic mixture, and the addition of heavy metals preclude FDA approval in the United States. We developed an ultra-small fluorescent protein in red, named smURFP, have optical properties similar to a small-molecule Cy5, subclass heptamethine cyanine dye (Ex / Em = 642/670 nm). smURFP have a fluorescence quantum results of 18% and express well in E. coli, which is the number of grams of fluorescent proteins purified from culture in the laboratory. In this study, combined with the smURFP fluorescent protein bovine serum albumin nanoparticles fluorescent protein. The fluorescent nanoparticles with quantum results of 17% and 12-14 nm in diameter.

Far-red fluorescent protein nanoparticles noninvasively image tumors in mice living through enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticles for in vivo fluorescent imaging. nanoparticle core of these proteins should prove useful as a scaffold biomacromolecular, which can bear extended chemical modifications to the study, such as imaging in vivo of nanoparticles fluorescent protein targeted to the primary cancer and metastases, treatment theranostic, and / or dual-modality imaging with positron emission tomography for entire human imaging.

Booster, Red-Shifted Genetic Encoding Forster Resonance Energy Transfer (FRET) Biosensor Compatible Cyan Fluorescent Protein / Yellow Fluorescent Protein FRET-based biosensor and Blue Light-Responsive Optogenetic Tools.

Genetically encoded Forster resonance energy transfer (FRET) based biosensor has been developed for the visualization of molecular activity signal. At present, most of them consisting of cyan and yellow fluorescent proteins (CFP and YFP), preclude the use of some of the FRET biosensor in one cell. In addition, the FRET biosensor based CFP and YFP is not compatible with optogenetic tools that operate on light blue. To resolve this problem, here, we have developed a FRET biosensor with red-shifted excitation and emission wavelengths.

Recombinant (E. coli) Red Fluorescent Proteins (RFP/dsRed) protein for ELISA or Standards (>98%)
RFP15-R 25 ug
EUR 300

Red Fluorescent Protein (RFP) Antibody
abx240273-100l 100 µl Ask for price

Red Fluorescent Protein (RFP) Antibody
abx240273-50l 50 µl
EUR 350

Red Fluorescent Protein (RFP) Antibody
abx412396-100g 100 µg
EUR 450

Red Fluorescent Protein (RFP) Antibody
abx412396-50ug 50 ug
EUR 678

Anti-RFP (Red Fluorescent Protein) Antibody
GWB-3BF397 100 ug Ask for price

Red Fluorescent Protein (RFP)
MBS6508207-01mg 0.1mg
EUR 770

Red Fluorescent Protein (RFP)
MBS6508207-5x01mg 5x0.1mg
EUR 3320

Native Red Fluorescent Protein
R-PE-004R 1mg
EUR 398.4
Description: Store R-PE in dark at ~ 2-8 centigrade. Do not freeze

Rabbit anti Red Fluorescent Protein
MBS225348-005mg 0.05mg
EUR 460

Rabbit anti Red Fluorescent Protein
MBS225348-5x005mg 5x0.05mg
EUR 1905

Red Fluorescent Protein (RFP) (AP)
MBS6497151-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (AP)
MBS6497151-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (PE)
MBS6497161-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (PE)
MBS6497161-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (APC)
MBS6497152-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (APC)
MBS6497152-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (HRP)
MBS6497155-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (HRP)
MBS6497155-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (FITC)
MBS6497154-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (FITC)
MBS6497154-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (Biotin)
MBS6497153-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (Biotin)
MBS6497153-5x01mL 5x0.1mL
EUR 4620

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
ABP-MAB-RT008 100 ug Ask for price

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-0025mg 0.025mg
EUR 220

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-005mg 0.05mg
EUR 260

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-01mg 0.1mg
EUR 325

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-02mg 0.2mg
EUR 450

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
MBS525008-05mg 0.5mg
EUR 705

RFP | Red Fluorescent Protein (clone RF5R)
AS15-3028 50 µg
EUR 259

Red Fluorescent Protein (RFP) (MaxLight 405)
MBS6497156-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 405)
MBS6497156-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 490)
MBS6497157-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 490)
MBS6497157-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 550)
MBS6497158-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 550)
MBS6497158-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 650)
MBS6497159-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 650)
MBS6497159-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein (RFP) (MaxLight 750)
MBS6497160-01mL 0.1mL
EUR 1060

Red Fluorescent Protein (RFP) (MaxLight 750)
MBS6497160-5x01mL 5x0.1mL
EUR 4620

Red Fluorescent Protein, Pre-adsorbed (RFP)
MBS6506872-01mg 0.1mg
EUR 715

Red Fluorescent Protein, Pre-adsorbed (RFP)
MBS6506872-5x01mg 5x0.1mg
EUR 3075

RFP | Red Fluorescent Protein (mouse monoclonal)
AS15-3033 100 µg
EUR 331

Recombinant Red Fluorescent Protein, His-tagged
RFP-005E 100µg
EUR 686.4
Description: At -20 centigrade.
At -80 centigrade for long-term storage.

Anti-GFP (Green Fluorescent Protein) (Mouse) Texas Red Conjugated Antibody
GWB-D2D74D 1 mg Ask for price

Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-1 each
EUR 164.4

Red Fluorescent Protein - R-PE (R-Phycoerythrin)
6005-5 each
EUR 326.4

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgBaculovirus 0.05mg(Baculovirus)
EUR 1065

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgEColi 0.05mg(E-Coli)
EUR 730

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-005mgYeast 0.05mg(Yeast)
EUR 895

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-02mgEColi 0.2mg(E-Coli)
EUR 975

Recombinant Discosoma sp. Red fluorescent protein drFP583
MBS1417453-05mgEColi 0.5mg(E-Coli)
EUR 1070

Fluorescent Protein Antibody Set
K817-6-100 each
EUR 1488

Green Fluorescent Protein Antibody
GWB-A6ABAF 0.25 mg Ask for price

Green Fluorescent Protein Antibody
GWB-21E341 1 ml Ask for price

Green Fluorescent protein Antibody
GWB-6A6C1C 0.1 mg Ask for price

Green Fluorescent Protein Antibody
GWB-2DAA70 0.1 ml Ask for price

Green Fluorescent Protein Antibody
GWB-B77B66 0.05 mg Ask for price

Green Fluorescent Protein Antibody
GWB-B8A68D 0.1 mg Ask for price

Green Fluorescent Protein Antibody
GWB-FF1300 0.1 mg Ask for price

Green Fluorescent Protein, X-adsorbed (GFP) (Texas Red)
MBS610666-1mg 1mg
EUR 1145

Green Fluorescent Protein, X-adsorbed (GFP) (Texas Red)
MBS610666-5x1mg 5x1mg
EUR 4990

Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-002mgEColi 0.02mg(E-Coli)
EUR 740

Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-002mgYeast 0.02mg(Yeast)
EUR 905

Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-01mgEColi 0.1mg(E-Coli)
EUR 885

Recombinant Entacmea quadricolor Red fluorescent protein eqFP611
MBS1437471-01mgYeast 0.1mg(Yeast)
EUR 1055

Blue Fluorescent Protein (BFP) Antibody
abx412394-100tests 100 tests
EUR 375

Blue Fluorescent Protein (BFP) Antibody
abx412394-50ug 50 ug
EUR 594

Cyan Fluorescent Protein (CFP) Antibody
abx412395-50ug 50 ug
EUR 594

Anti-GFP (Green Fluorescent Protein) (Goat) Texas Red Conjugated
GWB-2921F1 1 mg Ask for price

Rapid antibody fluorescent labeling kit (Texas Red)
EGQ00411 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (Texas Red)
MBS8584934-01mg 0.1mg
EUR 305

Rapid antibody fluorescent labeling kit (Texas Red)
MBS8584934-5x01mg 5x0.1mg
EUR 1480

Green Fluorescent Protein (GFP) Antibody
abx011187-100g 100 µg Ask for price

Green Fluorescent Protein (GFP) Antibody
abx011187-10g 10 µg
EUR 350

Green Fluorescent Protein (GFP) Antibody
abx011187-200g 200 µg Ask for price

Green Fluorescent Protein (GFP) Antibody
abx005584-100g 100 µg
EUR 200

Green Fluorescent Protein (GFP) Antibody
20-abx005584
  • Ask for price
  • Ask for price
  • Ask for price
  • 50 ul
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody
abx005585-100l 100 µl
EUR 200

Green Fluorescent Protein (GFP) Antibody
20-abx005585
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody
abx015749-100ul 100 ul
EUR 493.2

Green Fluorescent Protein (GFP) Antibody
abx015749-1mg 1 mg
EUR 362.5

Green Fluorescent Protein (GFP) Antibody
abx019093-100l 100 µl
EUR 243.75

Green Fluorescent Protein (GFP) Antibody
abx019093-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody
abx019094-100ug 100 ug
EUR 385.2

Green Fluorescent Protein (GFP) Antibody
abx019094-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody
abx019094-80l 80 µl
EUR 243.75

Green Fluorescent Protein (GFP) Antibody
abx019099-100ug 100 ug
EUR 410.4

Green Fluorescent Protein (GFP) Antibody
abx019099-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody
abx019099-80l 80 µl
EUR 300

Green Fluorescent Protein (GFP) Antibody
20-abx020016
  • Ask for price
  • Ask for price
  • 50 ug
  • 100 ug

Green Fluorescent Protein (GFP) Antibody
abx020016-400l 400 µl
EUR 387.5

Green Fluorescent Protein (GFP) Antibody
abx020016-80l 80 µl
EUR 300

Green Fluorescent Protein (GFP) Antibody
abx034623-100g 100 µg
EUR 281.25

Green Fluorescent Protein (GFP) Antibody
abx034623-400ul 400 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody
abx034623-80l 80 µl
EUR 343.2

Green Fluorescent Protein (GFP) Antibody
abx034624-100g 100 µg
EUR 525

Green Fluorescent Protein (GFP) Antibody
abx034624-100ul 100 ul
EUR 627.6

Green Fluorescent Protein (GFP) Antibody
abx037713-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody
abx037713-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody
abx037714-100ug 100 ug
EUR 469.2

Green Fluorescent Protein (GFP) Antibody
abx037714-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody
abx118007-100l 100 µl Ask for price

Green Fluorescent Protein (GFP) Antibody
20-abx118007
  • Ask for price
  • Ask for price
  • 50 ul
  • 100 ul

Green Fluorescent Protein (GFP) Antibody
abx118007-20l 20 µl
EUR 287.5

Green Fluorescent Protein (GFP) Antibody
abx118007-50l 50 µl
EUR 362.5

Green Fluorescent Protein (GFP) Antibody
abx132159-100tests 100 tests
EUR 275

Green Fluorescent Protein (GFP) Antibody
20-abx132159
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Green Fluorescent Protein (GFP) Antibody
20-abx134154
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody
20-abx134155
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody
20-abx134156
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody
20-abx134157
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

Green Fluorescent Protein (GFP) Antibody
abx137422-100tests 100 tests
EUR 225

Green Fluorescent Protein (GFP) Antibody
20-abx137422
  • Ask for price
  • Ask for price
  • Ask for price
  • 5 ug
  • 20 ug
  • 100 ug

Green Fluorescent Protein (GFP) Antibody
abx179079-96tests 96 tests
EUR 325

Green Fluorescent Protein (GFP) Antibody
abx140045-01mg 0.1 mg
EUR 460.8

Green Fluorescent Protein (GFP) Antibody
abx140045-100g 100 µg
EUR 300

Green Fluorescent Protein (GFP) Antibody
abx159519-100g 100 µg Ask for price

Green Fluorescent Protein (GFP) Antibody
20-abx159519
  • Ask for price
  • Ask for price
  • 50 ug
  • 100 ug

Green Fluorescent Protein (GFP) Antibody
abx159519-10g 10 µg
EUR 187.5

Green Fluorescent Protein (GFP) Antibody
abx159519-50g 50 µg
EUR 250

Green Fluorescent Protein (GFP) Antibody
abx341010-100l 100 µl
EUR 275

Green Fluorescent Protein (GFP) Antibody
abx341010-50l 50 µl
EUR 212.5

Green Fluorescent Protein (GFP) Antibody
abx233433-100g 100 µg
EUR 350

Green Fluorescent Protein (GFP) Antibody
abx233433-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody
abx233434-100g 100 µg
EUR 350

Green Fluorescent Protein (GFP) Antibody
abx233434-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody
abx233435-100ug 100 ug
EUR 577.2

Green Fluorescent Protein (GFP) Antibody
abx210090-100l 100 µl
EUR 275

Green Fluorescent Protein (GFP) Antibody
20-abx210090
  • Ask for price
  • Ask for price
  • 50 ug
  • 100 ug

Green Fluorescent Protein (GFP) Antibody
abx210090-50l 50 µl
EUR 212.5

Green Fluorescent Protein (GFP) Antibody
abx210351-100l 100 µl
EUR 350

Green Fluorescent Protein (GFP) Antibody
20-abx210351
  • Ask for price
  • Ask for price
  • 50 ul
  • 100 ul

Green Fluorescent Protein (GFP) Antibody
abx210351-50l 50 µl
EUR 250

Green Fluorescent Protein (GFP) Antibody
abx375279-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP) Antibody
abx375281-96tests 96 tests
EUR 337.5

Green Fluorescent Protein (GFP), Antibody
GWB-6E6630 1 mg Ask for price

Green Fluorescent Protein (GFP), Antibody
GWB-35A90C 0.1 mg Ask for price

Fluorescent Protein Antibody Set, BioAssay
MBS629957-6x01mg 6x0.1mg
EUR 1530

Green Fluorescent Protein (GFP) -HRP, Antibody
GWB-E12DB1 1 mg Ask for price

Anti- Green Fluorescent Protein (GFP) Antibody
GWB-6A60F0 0.2 mg Ask for price

Anti- Green Fluorescent Protein (GFP) Antibody
GWB-772B93 1 mg Ask for price

Green Fluorescent Protein (GFP) Antibody (FITC)
abx019260-100ug 100 ug
EUR 594

Green Fluorescent Protein (GFP) Antibody (FITC)
abx019260-400l 400 µl Ask for price

Green Fluorescent Protein (GFP) Antibody (FITC)
abx019260-80l 80 µl
EUR 425

Green Fluorescent Protein (GFP) Antibody Pair
abx370572-100g 100 µg
EUR 2725

Green Fluorescent Protein (GFP) Antibody Pair
20-abx370572
  • Ask for price
  • Ask for price
  • 5 × 96 tests
  • 10 × 96 tests

Green Fluorescent Protein (GFP) Antibody Pair
abx370572-50g 50 µg
EUR 1687.5

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-005mg 0.05mg
EUR 320

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-01mg 0.1mg
EUR 410

Recombinant Sea anemone Red fluorescent protein drFP583 protein, N- HN Tag
MBS157718-5x01mg 5x0.1mg
EUR 1520

Anti-GFP(Green fluorescent protein) Antibody
Y050794 100 µg
EUR 450

Enhanced Blue Fluorescent Protein (EBFP) Antibody
abx332838-100g 100 µg Ask for price

Enhanced Blue Fluorescent Protein (EBFP) Antibody
abx332838-20g 20 µg
EUR 362.5

Enhanced Blue Fluorescent Protein (EBFP) Antibody
abx332838-50g 50 µg Ask for price

We chose mKOκ and mKate2 as favorable donor and acceptor pair by calculating the distance Forster. By optimizing the fluorescent protein sequence and domain modulator of FRET biosensor, we developed a FRET biosensor backbone named “Booster”. Performance of protein kinase A (PKA) biosensor based backbone Booster (Booster-PKA) is comparable to AKAR3EV, a previously developed FRET biosensor consists CFP and YFP.